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RT-qPCR Related Discussions
  1. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)
  2. How to minimize delta ct value when everything else worked well in qRT PCR - (reply: 1)
  3. Pre-designed qRT-PCR primers - (reply: 1)
  4. Gene expression in colonic tissues using qRT-PCR - (reply: 1)
  5. t-test & qRT-PCR - (reply: 1)
  6. Contamination in qRT-PCR RT-minus - band similar to cDNA, not sure what it is? - (reply: 2)
  7. Gene expression varying over time course when it shouldn't (qRT-PCR) - (reply: 6)
  8. different amount of cDNA in qRT-PCR for target and reference genes - (reply: 3)
  9. RNA concentration is too low to detect gene of interest by qRT-PCR - (reply: 4)
  10. qRT-PCR works one day, and not the next - (reply: 1)
  11. Inter run calibration qRT-PCR - (reply: 2)
  12. ABI 7300 qRT-PCR and ddCT protocol - (reply: 1)
  13. Normalization of qRT-PCR following nuclear/cytoplasmic fractionation - (reply: 4)
  14. RT-qPCR primer problem - (reply: 4)
  15. Optimizing reactions for qRT-PCR using regular thermocycler - (reply: 4)
  16. High GC content RT-qPCR - please help - (reply: 3)
  17. qRT-PCR data analysis - (reply: 2)
  18. RT-qPCR normalization - (reply: 3)
  19. What is a use of ΔCt and ΔΔCt in qRT PCR? - (reply: 4)
  20. RT-qPCR plate set up NoRT controls - (reply: 1)
  21. Experiment plan for RT-qPCR internal reference search - (reply: 3)
  22. Experimental set up for qRT PCR - (reply: 13)
  23. Is some amount of RNA obligatory for qRT-PCR? - (reply: 5)
  24. Very late CP for control in rt-qPCR from RNAlater samples - (reply: 3)
  25. real time qRT-PCR and low expression gene - (reply: 2)
  26. Sybr green RT-qPCR primer - (reply: 1)
  27. Sybr green RT-qPCR primer - (reply: 1)
  28. Improving qRT-PCR detection limits - (reply: 2)
  29. Sequencing miRNA qRT-PCR Products - (reply: 5)
  30. Using DNA as a reference in qRT-PCR - (reply: 1)
  31. Real Easy qRT-PCR tutorial Links? - Links? (reply: 2)
  32. negative control in qRT-PCR - (reply: 6)
  33. RT-qPCR Experimental Design - How to arrange experimental replicates for treatment and control (reply: 1)
  34. Questions of Standards making in relative qRT-PCR - (reply: 2)
  35. relative comparison of many samples and genes in qRT-PCR - (reply: 1)
  36. Determine exon and intron for primer design - How to determine exon and intron for qRT-PCR primer design (reply: 2)
  37. qRT-PCR for plant gene expression - qRT-PCR for plant gene expression (reply: 5)
  38. PCR analysis method- delta or delta delta? - RNA, qRT PCR, delta Ct method (reply: 4)
  39. a question about 16s rRNA as reference in RT-qPCR - (reply: 1)
  40. question about qRT-PCR preparation - (reply: 1)
  41. General qRT-PCR question - (reply: 3)
  42. qRT-PCR to differentiate 3 Isoforms of 1 Gene - (reply: 1)
  43. Relative gene expression - Newbie at RT-qPCR! (reply: 3)
  44. qRT-PCR Inconsistency - (reply: 1)
  45. Workflow of qRT-PCR for siRNA - help please. (reply: 5)
  46. How to Normalize of RT-qPCR with several reference genes ? - (reply: 2)
  47. qRT-PCR Product Size - (reply: 5)
  48. qrt-pcr and northern blot ,which way is better? - (reply: 4)
  49. microrna quantitation ---use northern blot or qrt-pcr？ - (reply: 1)
  50. confirming an siRNA gene knockdown using qRT-PCR - (reply: 1)
  51. Amplify cDNA for qRT-PCR? - (reply: 6)
  52. qRT-PCR no of cycles?? - (reply: 6)
  53. Is my primer going to work for qRT-PCR? - (reply: 6)
  54. Cell-cycle independant qRT-PCR internal control - anyone has experience doing this? (reply: 1)
  55. Ncode qRT-PCR - (reply: 6)
  56. qRT-PCR calculations - Averaging across biological replicates (reply: 2)
  57. Endogenous control in bacterial RT-qPCR - (reply: 4)
  58. qRT-PCR Problems - (reply: 1)
  59. qRT-PCR with DMSO - (reply: 2)
  60. Problems with qRT-PCR - (reply: 3)
  61. Optimizing multiplex RT-qPCR - (reply: 3)