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Protocols
- RNA Interference (RNAi) in Cultured Cells (Drosophila RNAi Screening Center at Harvard Medical School)
The protocols listed here are for Drosophila cells in 6 well plates and our pre-aliquoted 384 well plates. RNAi experiments may be done in other size plates, just scale up or down.
http://flyrnai.org/DRSC-PRR.html
Added: Wed May 12 2004, Hits: 3855, Reviews: 0 Write review Cached -
Lymphocyte siRNA electroporation transfection
(McManus Lab, UCSF)
http://www.google.com/url?sa=t&source=web&ct=res&c...
Added: Thu Feb 26 2009, Hits: 1329, Reviews: 0 Write review -
Optimize siRNA Transfection
(Mirus Bio, LLC)
Tips on optimizing siRNA transfection conditions
http://www.mirusbio.com/tech_resources/tips/optimi...
Added: Tue Mar 12 2013, Hits: 849, Reviews: 0 Write review -
Reverse Transfection of Adherent Cells with siRNA
(Berkeley Screening Center)
http://bsc.berkeley.edu/index.php/recommended-prot...
Added: Thu Feb 26 2009, Hits: 1086, Reviews: 0 Write review Cached -
siRNA reverse transfection protocol with RNAiMax (siTOOLs Biotech)
(siTOOLs Biotech)
This protocol can be used to reverse transfect siPOOLs or other siRNA reagents into standard/primary cell lines using RNAiMax. For forward transfection, seed cells prior to transfection.
https://www.sitoolsbiotech.com/pdf/siPool%20Transf...
Added: Fri Aug 04 2017, Hits: 221, Reviews: 0 Write review -
siRNA Transfection Protocol
(Cell Signaling)
http://www.cellsignal.com/support/protocols/siRNA....
Added: Thu Feb 26 2009, Hits: 4441, Reviews: 0 Write review -
Tandem siRNA transfection
(Lykke-Andersen Lab, Department of MCD Biology, University of Colorado)
Sequential siRNA transfection
http://www.colorado.edu/mcdb/Lykke_Andersen/update...
Added: Thu Feb 26 2009, Hits: 1442, Reviews: 0 Write review Cached