Top : Molecular Biology : RNA : RNA Extraction and Isolation

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  • Solution recipes for the buffers in Qiagen kits (Kevin William)
    DNA or RNA kits are so expensive now. You can make your own kits by bulk RNA and DNA spin columns with home made solutions or buy bulk solutions.
    http://openwetware.org/wiki/Economic_substitute_fo...
    Added: Tue Oct 29 2013, Hits: 1589, Reviews: 0  Write review  
  • Total RNA isolation protocol (Evrogen)
    The procedure is suitable for all types of tissues from wide variety of animal species. The method is based on the well-known protocol of Chomczynski and Sacchi (Chomczynski and Sacchi, 1987), except that all steps are performed at neutral pH instead of acidic as was originally suggested.
    http://www.evrogen.com/technologies/RNA-isolation....
    Added: Fri Feb 06 2009, Hits: 3787, Reviews: 0  Write review   Cached
  • RNA and protein extraction from the same tissue (Ivan J Delgado)
    http://ivaan.com/protocols/139.html
    Added: Sun Mar 01 2009, Hits: 6087, Reviews: 0  Write review   Cached
  • DNA/RNA Extraction from the Same Material (Pat Heslop-Harrison, Molecular Cytogenetics, University of Leicester)
    A protocol for extraction of both DNA and RNA from the same material typically plant leaves.
    http://www.le.ac.uk/biology/phh4/dnarna.htm
    Added: Fri Feb 06 2009, Hits: 4468, Reviews: 0  Write review   Cached
  • How to prepare Molecular Biology grade glycogen (Contributed by Roberto Salvi)
    Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors, the main disadvantage is that it is very expensive (e.g., About 100 dollars/20-40 mg). Here we present a simple and inexpensive protocol to prepare a large amount of glycogen which is suitable for any kind of Molecular Biology application (we routinely use it for RNA work).
    Added: Tue May 14 2002, Reviews: 0  Write review  
  • Single Step RNA Isolation PDF file (Steven Finkbeiner, UCSF)
    http://www.gladstone.ucsf.edu/gladstone/files/fink...
    Added: Fri Feb 20 2009, Hits: 2456, Reviews: 0  Write review