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Protocols
- Acid-Guanidinium Thiocyanate-Phenol-Chloroform RNA Purification (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
This method describes preparation from small quantities (~ 50mg) of tissue. Appropriate scaling of the volumes involved can be performed to accomodate larger quantities.
http://www.nottingham.ac.uk/~mbzspd/methods/Acid-g...
Added: Sat Apr 10 2004, Hits: 3923, Reviews: 0 Write review Cached -
Acid Phenol Method for Isolation of RNA from Adult Mouse Tissues
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 2442, Reviews: 0 Write review Cached -
C. Elegans RNA Isolation
(Ambros Lab)
http://www.dartmouth.edu/artsci/bio/ambros/protoco...
Added: Tue May 14 2002, Hits: 1240, Reviews: 0 Write review Cached -
Drosophila RNA Preparation
(Vesicle Trafficking, Stanford University)
http://cmgm.stanford.edu/~vesicle/protocols/dros_r...
Added: Thu Jul 25 2002, Hits: 1571, Reviews: 0 Write review Cached -
Increasing Your RNA Recovery
(Ambion)
Provides very useful information on sample treatment for RNA extraction. Three method are described for preparing tissue lysis.
http://www.ambion.com/techlib/tips/increasing-reco...
Added: Tue May 14 2002, Hits: 2712, Reviews: 0 Write review Cached -
Isolation of total cellular RNA from brain tissue
(Practical Approach Online, Oxford University Press)
Isolating RNA using Trizol reagent
http://www.oup.co.uk/pdf/pas/5-7-2.pdf
Added: Sat Aug 17 2002, Hits: 3318, Reviews: 0 Write review -
LiCl RNA Preparation
(Ambros Lab)
http://www.dartmouth.edu/artsci/bio/ambros/protoco...
Added: Tue May 14 2002, Hits: 1119, Reviews: 0 Write review Cached -
RNA Extraction with TRIZOL
(Bissell Lab, Berkely Lab)
http://www.lbl.gov/LBL-Programs/lifesciences/Bisse...
Added: Sat Feb 21 2009, Hits: 3549, Reviews: 0 Write review Cached -
RNA Isolation Using Guanidinium Hydrochloride
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
http://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 1550, Reviews: 0 Write review Cached -
Total Cellular RNA Isolation
(Faulkner-Jones)
Large scale total cellular RNA isolation using 4M guanidinium isothiocyanate lysis buffer and caesium chloride ultracentrifugation from adult organs.
http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
Added: Tue May 14 2002, Hits: 2385, Reviews: 0 Write review Cached -
Total RNA Isolation
(Carl Clark)
This is a guanidine-based method for isolating RNA from zebrafish embryos.
http://biosci.cbs.umn.edu/~kclark/protocols/totalR...
Added: Tue May 14 2002, Hits: 3469, Reviews: 0 Write review Cached -
Total RNA Isolation from Difficult Tissues
(Ambion)
Troubleshooting techniques to help overcome problems associated with isolating total RNA from difficult tissues.
http://www.ambion.com/techlib/tips/difficult-isola...
Added: Tue May 14 2002, Hits: 3115, Reviews: 0 Write review Cached -
Total RNA Isolation-the Lithium Chloride-SDS-Urea Method
(Faulkner-Jones)
This method can be used for some adult tissues, most cell lines and embryos. Harvested tissues are homogenised in urea-lithium and the relatively insoluble lithium-RNA salts are precipitated overnight at 4oC. Much of the DNA remains in solution. The recovered lithium-RNA is acid phenol-chloroform extracted to remove contaminating proteins...
http://grimwade.biochem.unimelb.edu.au/bfjones/gen...
Added: Tue May 14 2002, Hits: 940, Reviews: 0 Write review Cached -
Total RNA Preparation (from C. elegans)
(Ambros Lab)
http://www.dartmouth.edu/artsci/bio/ambros/protoco...
Added: Tue May 14 2002, Hits: 1033, Reviews: 0 Write review Cached -
Worm RNA Preparation
(Ambros Lab)
http://www.dartmouth.edu/artsci/bio/ambros/protoco...
Added: Tue May 14 2002, Hits: 553, Reviews: 0 Write review Cached