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DNase Treatment
(Practical Approach Online, Oxford University Press)
DNase treatment of total cellular RNA to remove contaminating genomic DNA
http://www.oup.co.uk/pdf/pas/5-7-4.pdf
Added: Sat Aug 17 2002, Hits: 490, Reviews: 0 Write review - How to prepare Molecular Biology grade glycogen (Contributed by Roberto Salvi)
Glycogen can conveniently substitute for tRNA as a carrier for nucleic acid precipitation. Although Molecular Biology grade glycogen can be purchased from a number of vendors, the main disadvantage is that it is very expensive (e.g., About 100 dollars/20-40 mg). Here we present a simple and inexpensive protocol to prepare a large amount of glycogen which is suitable for any kind of Molecular Biology application (we routinely use it for RNA work).
Added: Tue May 14 2002, Reviews: 0 Write review