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Protocols
- Acid-Guanidinium Thiocyanate-Phenol-Chloroform RNA Purification (Dr. Simon Dawson, Department of Biochemistry, University of Nottingham Medical School)
This method describes preparation from small quantities (~ 50mg) of tissue. Appropriate scaling of the volumes involved can be performed to accomodate larger quantities.
https://www.nottingham.ac.uk/~mbzspd/methods/Acid-g...
Added: Sat Apr 10 2004, Hits: 565, Reviews: 0
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Acid Phenol Method for Isolation of RNA from Adult Mouse Tissues
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
https://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 636, Reviews: 0
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C. Elegans RNA Isolation
(Ambros Lab)
https://www.dartmouth.edu/artsci/bio/ambros/protoco...
Added: Tue May 14 2002, Hits: 306, Reviews: 0
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Drosophila RNA Preparation
(Vesicle Trafficking, Stanford University)
https://cmgm.stanford.edu/~vesicle/protocols/dros_r...
Added: Thu Jul 25 2002, Hits: 265, Reviews: 0
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Increasing Your RNA Recovery
(Ambion)
Provides very useful information on sample treatment for RNA extraction. Three method are described for preparing tissue lysis.
https://www.ambion.com/techlib/tips/increasing-reco...
Added: Tue May 14 2002, Hits: 691, Reviews: 0
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Isolation of total cellular RNA from brain tissue
(Practical Approach Online, Oxford University Press)
Isolating RNA using Trizol reagent
https://www.oup.co.uk/pdf/pas/5-7-2.pdf
Added: Sat Aug 17 2002, Hits: 563, Reviews: 0
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LiCl RNA Preparation
(Ambros Lab)
https://www.dartmouth.edu/artsci/bio/ambros/protoco...
Added: Tue May 14 2002, Hits: 355, Reviews: 0
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RNA Isolation Using Guanidinium Hydrochloride
(Bowtell Lab Manual, Peter MacCallum Cancer Institute)
https://www.pmci.unimelb.edu.au/core_facilities/man...
Added: Tue May 14 2002, Hits: 419, Reviews: 0
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Total Cellular RNA Isolation
(Faulkner-Jones)
Large scale total cellular RNA isolation using 4M guanidinium isothiocyanate lysis buffer and caesium chloride ultracentrifugation from adult organs.
https://grimwade.biochem.unimelb.edu.au/bfjones/gen...
Added: Tue May 14 2002, Hits: 551, Reviews: 0
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Total RNA Isolation
(Carl Clark)
This is a guanidine-based method for isolating RNA from zebrafish embryos.
https://biosci.cbs.umn.edu/~kclark/protocols/totalR...
Added: Tue May 14 2002, Hits: 519, Reviews: 0
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Total RNA Isolation from Difficult Tissues
(Ambion)
Troubleshooting techniques to help overcome problems associated with isolating total RNA from difficult tissues.
https://www.ambion.com/techlib/tips/difficult-isola...
Added: Tue May 14 2002, Hits: 605, Reviews: 0
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Total RNA Isolation-the Lithium Chloride-SDS-Urea Method
(Faulkner-Jones)
This method can be used for some adult tissues, most cell lines and embryos. Harvested tissues are homogenised in urea-lithium and the relatively insoluble lithium-RNA salts are precipitated overnight at 4oC. Much of the DNA remains in solution. The recovered lithium-RNA is acid phenol-chloroform extracted to remove contaminating proteins...
https://grimwade.biochem.unimelb.edu.au/bfjones/gen...
Added: Tue May 14 2002, Hits: 202, Reviews: 0
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Total RNA Preparation (from C. elegans)
(Ambros Lab)
https://www.dartmouth.edu/artsci/bio/ambros/protoco...
Added: Tue May 14 2002, Hits: 207, Reviews: 0
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Worm RNA Preparation
(Ambros Lab)
https://www.dartmouth.edu/artsci/bio/ambros/protoco...
Added: Tue May 14 2002, Hits: 94, Reviews: 0
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