Protocols
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BAC&PAC Mini Prep
(BACPAC Resource Center at the Children's Hospital Oakland Research Institute )
This is a rapid alkaline lysis miniprep method for isolating DNA from large PAC clones. It is a modification of a standard Qiagen-Tip method that uses no organic extractions or columns. The method works very well for doing analytical restriction digests of PAC clones and can be scaled up if necessary.
http://bacpac.chori.org/bacpacmini.htm
Added: Tue Jul 30 2002, Hits: 901, Reviews: 0 Write review Cached -
Cycle Sequence Reactions For Large Insert Plasmid Templates
(BACPAC Resource Center at the Children's Hospital Oakland Research Institute )
The dye-labeled terminator reaction chemistries have been designed to balance conservation of reagents with the resulting sequence product signal strength.
http://bacpac.chori.org/cyclesere.htm
Added: Tue Jul 30 2002, Hits: 604, Reviews: 0 Write review Cached -
Preparation of BAC DNA Clones
(BACPAC Resource Center at the Children's Hospital Oakland Research Institute)
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmids
http://bacpac.chori.org/dnaprep.htm
Added: Tue Jul 30 2002, Hits: 723, Reviews: 0 Write review Cached