Top : Molecular Biology : DNA Sequencing : DNA Sequencing by the Chemical Method


Protocols
  • Chemical Sequencing of DNA (The Hahn Lab, The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute, Seattle)
    This is a rapid method for chemical DNA sequencing which is commonly used as ladder for footprinting reactions or for sequencing of short DNA oligonucleotides.
    http://labs.fhcrc.org/hahn/Methods/mol_bio_meth/ch...
    Added: Sat Mar 27 2004, Hits: 1543, Reviews: 0  Write review   Cached
  • DNA sequencing with 33P ddNTP (Atle M. Bones, The NTNU Plant Genetics Lab)
    http://boneslab.bio.ntnu.no/old_root/dnaseqman.htm
    Added: Sun Dec 05 2010, Hits: 466, Reviews: 0  Write review  
  • Maxam-Gilbert Sequencing PDF file (The Morimoto Lab, Northwestern University)
    A modified, abbreviated version of the Maxam-Gilbert sequencing is described.
    http://www.biochem.northwestern.edu/morimoto/resea...
    Added: Sat Mar 27 2004, Hits: 1391, Reviews: 0  Write review  
  • Preparation of G+A Marker ( Protocol Online)
    Simplified method for preparing G+A ladder run along with footprinting reaction. It's much simple than the original Maxima-Gilbert sequencing reaction and works fine.
    Added: Tue May 14 2002, Reviews: 0  Write review