Protocols
- Genomic DNA extraction
(CIMMYT Applied Molecular Genetics Laboratory)
Genomic DNA extraction from plant material using CTAB buffer, may also apply to other materials.
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Fri Aug 02 2002, Hits: 30055, Reviews: 0 Write review - A Rapid Method to Isolate Plant Genomic DNA (Atle M. Bones, Norwegian University of Science and Technology)
http://boneslab.bio.ntnu.no/old_root/quickprepdna....
Added: Tue May 14 2002, Hits: 11486, Reviews: 0 Write review Cached - Plant DNA Extraction Protocol (Lazo Lab, USDA)
A. thaliana has a very small haploid genome and this makes obtaining DNA somewhat difficult. The most notable problem is that DNA is usually contaminated with polysaccharide which inhibit restriction enzymes as well as other DNA modifying enzymes. This problem is most easily solved by using young plants which have not accumulated as much polysaccharide as older plants. The best results are obtained with plants that are two to three weeks post germinated.
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplm...
Added: Sun Mar 22 2009, Hits: 8710, Reviews: 0 Write review Cached - A High throughput DNA extraction method for plant tissues rich in polyphenols, terpenes and polysacc (Bronwyn Matheson)
A DNA extraction protocol for tannin rich Eucalyptus and Pinus samples which also works very well for Acacia, Populus, Oak and Cotton. This method is suitable for 96 well arrays and makes use of PVPP or PVP 360 to mop up poly-phenols.
Added: Tue Dec 07 2010, Reviews: 0 Write review -
Arabidopsis DNA Purification from Nuclei
(Lazo Lab, USDA)
http://wheat.pw.usda.gov/~lazo/methods/lazo/arabdn...
Added: Sun Mar 22 2009, Hits: 1150, Reviews: 0 Write review Cached -
Cetyltrimethylammonium bromide (CTAB) Plant DNA Extraction
(BioProtocol)
Detailed protocol for extracting DNA from leaves
http://bioprotocol.bio.com/protocolstools/protocol...
Added: Sun Jul 21 2002, Hits: 1360, Reviews: 0 Write review Cached -
Cotton DNA Isolation
(Lazo Lab, USDA)
Isolation of Cotton Genomic DNA from Leaf Tissue
http://wheat.pw.usda.gov/~lazo/methods/lazo/cotton...
Added: Sun Mar 22 2009, Hits: 1304, Reviews: 0 Write review Cached - Development of a rapid and efficient method for DNA extraction from plants containing high amount of (Protocol Online)
Extraction was repeated several times using chloroform: isoamylalchohol to obtain more purified DNA. Average yield of DNA extracted with this procedure was 700-1500 ng/microL from 100 mg fresh leaf tissue. Extracted DNA showed complete digestion with restriction endonuclease and successful reproducibility through PCR amplification. This method, which is relatively inexpensive, could be efficiently applied for other species of different families that contain polyphenols and secondary methabolite is cumbersome.
Added: Tue Jan 18 2011, Reviews: 0 Write review -
DNA and RNA Double Isolation from Plant Leaf
(Pat Heslop-Harrison and Trude Schwarzacher, Department of Biology, University of Leicester)
A protocol for extraction / isolation of both DNA and RNA from the same material typically plant leaf / leaves
http://www.le.ac.uk/biology/phh4/methods/dnarna.ht...
Added: Sat Mar 27 2004, Hits: 4914, Reviews: 0 Write review Cached -
DNA extraction using the 'sap extractor '
(CIMMYT Applied Molecular Genetics Laboratory)
http://www.cimmyt.org/english/docs/manual/protocol...
Added: Fri Aug 02 2002, Hits: 2553, Reviews: 0 Write review -
DNA Isolation from Plant Leaf
(Pat Heslop-Harrison and Trude Schwarzacher, Department of Biology, University of Leicester)
CTAB method
http://www.le.ac.uk/biology/phh4/methods/dnaiso.ht...
Added: Sat Mar 27 2004, Hits: 10379, Reviews: 0 Write review Cached -
DNA Isolation Procedures
(McFall-Ngai Lab, University of Wisconsin–Madison)
This manual is a must read which describes many classic protocols for isolating DNA from a variety of sources.
http://labs.medmicro.wisc.edu/mcfall-ngai/papers/2...
Added: Sat Dec 08 2012, Hits: 11284, Reviews: 0 Write review -
DNA Purification-Modified CTAB Procedure
(Kim Marshall)
DNA extraction from needles
http://dendrome.ucdavis.edu/Protocols/dna_from_nee...
Added: Tue May 14 2002, Hits: 810, Reviews: 0 Write review Cached -
Extraction DNA from Plant Materials
(IDT)
General plant DNA isolation protocol. Plant materials are among the most difficult for high quality DNA extractions. The key is to properly prepare the tissues for extraction. In most cases this involves the use of liquid nitrogen flash freezing followed by grinding the frozen tissue with a mortar and pestle.
http://www.idtdna.com/support/technical/TechnicalB...
Added: Sun Mar 22 2009, Hits: 9872, Reviews: 0 Write review -
Genomic Plant DNA Extraction for Mapping
(Kay Schneitz Lab)
This protocol is quick, reliable and the DNA obtained can be used for PCR-based mapping using SSLP and CAPS markers.
http://www.unizh.ch/botinst/Cyto_Website/schneitzL...
Added: Tue May 14 2002, Hits: 3151, Reviews: 0 Write review Cached -
Isolation of genomic DNA and total RNA from same total nucleic acid preparation
(Craig Pikaard's Laboratory, Indiana University)
this protocol allows you to isolate both genomic DNA and total RNA from the same tissue sample
http://sites.bio.indiana.edu/~pikaardlab/PDFs%20an...
Added: Tue Mar 03 2009, Hits: 1528, Reviews: 0 Write review Cached -
Mitochondria DNA Isolation
(Gerard Lazo Lab)
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplm...
Added: Fri Apr 09 2004, Hits: 2221, Reviews: 0 Write review Cached -
Plant Chloroplast DNA Extraction Protocol
(Lazo Lab, USDA)
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplc...
Added: Sun Mar 22 2009, Hits: 906, Reviews: 0 Write review Cached -
Plant Genomic DNA Extraction using CTAB
(ARC Centre of Excellence for Integrative Legume Research The University of Queensland)
DNA extraction from plant tissue can vary depending on the material used. Essentially any mechanical means of breaking down the cell wall and membranes to allow access to nuclear material, without its degradation is required. For this, usually an initial grinding stage with liquid nitrogen is employed to break down cell wall material and allow access to DNA while harmful cellular enzymes and chemicals remain inactivated. Once the tissue has been sufficiently ground, it can then be resuspended in a suitable buffer, such as CTAB...
http://www.cilr.uq.edu.au/UserImages/File/Plant%20...
Added: Sun Mar 22 2009, Hits: 10374, Reviews: 0 Write review -
Plant Nuclear DNA Preparation
(Lazo Lab, USDA)
This method can be used to prepare Petunia cv. Rose du Ciel nuclear DNA suitable for restriction enzyme analysis and cloning. Chloroplast DNA contamination will vary depending on the genotype. Greater chloroplast DNA contamination results when this method is used with the "Mitchell" line of Petunia.
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnaplp...
Added: Sun Mar 22 2009, Hits: 746, Reviews: 0 Write review Cached -
Plant Nuclei DNA Extraction Protocol
(Lazo Lab, USDA)
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnapln...
Added: Sun Mar 22 2009, Hits: 901, Reviews: 0 Write review Cached -
Plant Total DNA Isolation
(Byeong-Ha Lee at the University of Arizona )
describes a method for isolating DNA from plant tissue
http://bioprotocol.bio.com/protocolstools/protocol...
Added: Sun Jul 21 2002, Hits: 1170, Reviews: 0 Write review Cached -
Preparation of Megabase-size DNA
(Clemson University Genomics Institute)
To construct large insert DNA libraries in BAC and YAC vectors, methods must be developed to isolate very high molecular weight DNA - megabase-size DNA
http://www.genome.clemson.edu/groups/bac/protocols...
Added: Tue May 14 2002, Hits: 372, Reviews: 0 Write review Cached - Rapid DNA Isolation from Phyllanthus Amarus and Other Plant Tissues (Protocol Online)
Added: Mon Feb 02 2009, Reviews: 0 Write review -
Single leaf DNA prep for Southern blot analysis of transgenic plants in first generation
(Craig Pikaard's Laboratory, Indiana University)
http://sites.bio.indiana.edu/~pikaardlab/PDFs%20an...
Added: Tue Mar 03 2009, Hits: 788, Reviews: 0 Write review Cached