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Protocols
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Chromosomal DNA Isolation
(Hancock Laboratory Methods, Department of Microbiology and Immunology,University of British Columbia)
For bacteria chromosomal DNA
http://www.cmdr.ubc.ca/bobh/showmethod.php?methodi...
Added: Sat Aug 31 2002, Hits: 12326, Reviews: 0
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DNA Extraction from Bacteria
(Lazo Lab)
Phenol/chloroform method
http://wheat.pw.usda.gov/~lazo/methods/lazo/dnagen...
Added: Tue May 14 2002, Hits: 12052, Reviews: 0
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DNA Extraction from Bacteria
(Julie B. Wolf, University of Maryland, Baltimore County)
Phenol/chloroform method
http://www.research.umbc.edu/~jwolf/m1.htm#genomic...
Added: Tue May 14 2002, Hits: 18389, Reviews: 0
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DNA Extraction From Bacteria (Triton Method)
(NWFSC)
http://micro.nwfsc.noaa.gov/protocols/tritonprep.h...
Added: Tue May 14 2002, Hits: 10533, Reviews: 0
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DNA Extraction from Gram-positive Bacteria
(NWFSC)
This procedure was originally developed for Listeria monocytogenes but has worked well with other Gram+ bacteria
http://micro.nwfsc.noaa.gov/protocols/GramPosDNA.h...
Added: Tue May 14 2002, Hits: 9137, Reviews: 0
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E.coli Total RNA Isolation
(E. coli Genome Project at UW-Madison)
Stablize E.coli RNA with Qiagen Bacteria RNA Protect Reagent and isolate total RNA with Epicentre MasterPure RNA Purification Kit. Extract RNA for microarray
http://www.genome.wisc.edu/resources/protocols/rna...
Added: Mon Aug 05 2002, Hits: 4251, Reviews: 0
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Genomic DNA Isolation
(Jeffrey D. Newman, Lycoming College)
Phenol/chloroform method, this procedure does not work well with Gram + cocci
http://srv2.lycoming.edu/~newman/rrna/gdnaisol.htm
Added: Tue May 14 2002, Hits: 7541, Reviews: 0
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Genomic DNA isolation from Bacteria
(Newman Lab)
http://srv2.lycoming.edu/~newman/rrna/gdnaisol.htm
Added: Tue May 14 2002, Hits: 9187, Reviews: 0
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Isolation and Quantification of Genomic DNA from Mycobacterium tuberculosis
(The Northeast Regional RFLP Laboratory for DNA Typing)
This protocol describes a "DNA mini-prep" procedure for the isolation of chromosomal DNA from Mycobacterium tuberculosis and a procedure for quantifying the amount isolated
http://www.wadsworth.org/rflp/Tutorials/DNAisolati...
Added: Tue May 14 2002, Hits: 527, Reviews: 1 Read reviews
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Isolation Of RNA From Bacteroids and Bacteria
(Lazo Lab)
http://wheat.pw.usda.gov/~lazo/methods/lazo/bactrn...
Added: Tue May 14 2002, Hits: 1857, Reviews: 0
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Pulsed-field Gel Purification of E. coli Restriction Fragments
(Stanford DNA Sequence & Technology Center)
http://sequence-www.stanford.edu/protocols/pulsed-...
Added: Tue May 14 2002, Hits: 1536, Reviews: 0
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