Protocols
-
Chromatin Assembly Protocol
(Tsukiyama Lab, Fred Hutchinson Cancer Research Center)
http://labs.fhcrc.org/tsukiyama/protocols/chromati...
Added: Tue Mar 03 2009, Hits: 1069, Reviews: 0 Write review -
Chromatin Electrophoresis
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt10/ex10...
Added: Tue May 14 2002, Hits: 1039, Reviews: 0 Write review Cached -
Expression, Purification and Reconstitution of Recombinant Yeast Histones
(Tsukiyama Lab, Fred Hutchinson Cancer Research Center)
http://labs.fhcrc.org/tsukiyama/protocols/expressi...
Added: Tue Mar 03 2009, Hits: 806, Reviews: 0 Write review -
Extraction and Electrophoresis of Histones
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt10/ex10...
Added: Tue May 14 2002, Hits: 1048, Reviews: 0 Write review Cached -
Extraction of Chromatin
(Dr. William H. Heidcamp, Biology Department, Gustavus Adolphus College)
http://homepages.gac.edu/~cellab/chpts/chpt10/ex10...
Added: Tue May 14 2002, Hits: 1138, Reviews: 0 Write review Cached -
Pelleting Assembled Chromatin for Microscopy
(Mitchison Lab)
http://mitchison.med.harvard.edu/protocols/chr2.ht...
Added: Tue May 14 2002, Hits: 439, Reviews: 0 Write review Cached -
Peptide Pull-Down (PPD) Assay for Identification and Characterization of Histone PTM Effectors
(Andrew K. Roosand Joanna Wysocka)
To comprehend the complexity of epigenetic regulation, it is essential to not only catalogue histone PTMs and their patterns, but also to understand roles that histone PTMs and their effectors play in biological processes. An important component of this understanding will come through identification of histone PTM binding proteins. To this end, the peptide pull-down (PPD) assay provides a simple and effective tool to identify and characterize such reader proteins
http://www.epigenome-noe.net/researchtools/protoco...
Added: Thu Feb 25 2010, Hits: 264, Reviews: 0 Write review Cached -
Purification of core histones from Broccoli
(Craig Pikaard's Laboratory, Indiana University)
http://sites.bio.indiana.edu/~pikaardlab/PDFs%20an...
Added: Tue Mar 03 2009, Hits: 1041, Reviews: 0 Write review -
Purification of Human Multiprotein Complexes using OneSTrEP Technology
(Epigenome Network of Excellence)
A strategy for isolation of multiprotein complexes from human HeLa S3 cells in a scale and purity optimized for characterization by mass spectrometry, using stably expressed One-STrEP-tag ® fusion proteins.
http://www.epigenome-noe.net/researchtools/protoco...
Added: Wed May 27 2009, Hits: 208, Reviews: 0 Write review Cached -
Quick Chromatin Digestions
(Tsukiyama Lab, Fred Hutchinson Cancer Research Center)
http://labs.fhcrc.org/tsukiyama/protocols/quick_ch...
Added: Tue Mar 03 2009, Hits: 872, Reviews: 0 Write review