Top : Cell Biology : Flow Cytometry (FCM)

Summary: Flow cytometry (FCM) is a means of measuring certain physical and chemical characteristics of cells or particles as they pass in a fluid stream by a beam of laser light. The term "flow cytometry" derives from the measurement (meter) of single cells (cyto) as they flow past a series of detectors. Flow Sorting extends flow cytometry by using electrical or mechanical means to divert and collect cells with one or more measured characteristics falling within a range or ranges of values set by the user. The major applications of FCM include the analysis of cell cycle, apoptosis, necrosis, multicolor analysis, cell sorting, functional analysis and stem cell analysis, etc.
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Protocols
  • Complete Guide to Flow Cytometry (FACS) for Cell Cycle PDF file (Andrew Burgess, PhD)
    A complete Guide to Cell Cycle FACS, detailing how to fix, stain, aquire and analyze cells using a BD FACSCalibur and CellQuest software.
    http://sciencetechblog.files.wordpress.com/2011/05...
    Added: Wed May 25 2011, Hits: 4972, Reviews: 0  Write review  
  • Complete Guide to Flow Cytometry for Cell Cycle PDF file (Andrew Burgess, PhD)
    A complete Guide detailing how to fix, stain, aquire and analyze cells using a BD FACSCalibur and CellQuest software
    http://www.sciencetechblog.com/ScienceTechBlog/Sci...
    Added: Thu Jun 10 2010, Hits: 12366, Reviews: 0  Write review  
  • FACS Troubleshootings (Protocol Online)
    Archives of FACS troubleshootings
    http://www.protocol-online.org/biology-forums/flow...
    Added: Wed Feb 11 2009, Hits: 3756, Reviews: 0  Write review   Cached
  • Flow Cytometry Analysis (Springer Lab, Harvard University)
    Flow cytometry employs instrumentation that scans single cells flowing past excitation sources in a liquid medium. The technology can provide rapid, quantitative, multiparameter analyses on single living (or dead) cells based on the measurement of visible and fluorescent light emission. Flow cytometry is a widely used method for characterizing and separating individual cells. This basic protocol focuses on: measure fluorescence intensity produced by fluorescent-labled antibodies and ligands that bind specific cell-associated molecules.
    http://www.cbrinstitute.org/labs/springer/protocol...
    Added: Wed Aug 14 2002, Hits: 5615, Reviews: 0  Write review   Cached
  • Advanced Methodologies in Flow Cytometry (Cytomety Lab, Purdue Univ)
    A large collection of methodological paper on cytometry
    http://www.cyto.purdue.edu/flowcyt/research/cytote...
    Added: Tue May 14 2002, Hits: 3682, Reviews: 0  Write review   Cached
  • Direct Immunofluorescence Staining (U of Florida Flow Cytometry Core Laboratory)
    This is the method for direct immunofluorescence labeling; that is, the antibodies have the fluorescent dye attached. Direct labeling is simpler and quicker than indirect labeling.
    http://www.biotech.ufl.edu/FlowCytometry/direct.ht...
    Added: Tue May 14 2002, Hits: 1339, Reviews: 0  Write review   Cached
  • Flow Cytometry: Indirect Immunofluorescence Staining of Human Platelets (BD Pharmingen)
    Protocol for general use.
    http://www.bdbiosciences.com/pharmingen/protocols/...
    Added: Sat Jul 27 2002, Hits: 2178, Reviews: 0  Write review   Cached
  • Fluorescein Labeling (David Chambers)
    http://pingu.salk.edu/flow/protocols/flabel.html
    Added: Tue May 14 2002, Hits: 1818, Reviews: 0  Write review   Cached
  • Fluorescence and Flow Cytometry Tutorials Video (Invitrogen )
    These tutorials provide a great overview of the basics of fluorescence and flow cytometry, covering the theory and instrumentation and are a great resource for new students or researchers.
    http://www.invitrogen.com/site/us/en/home/support/...
    Added: Tue May 18 2010, Hits: 2053, Reviews: 0  Write review  
  • Immunofluorescent Staining for FACS Analysis (Flow Cytometry Core Facility, PrincetonUniversity)
    General immunofluorescence staining protocol using directly and indirectly conjugated antibodies
    http://www.molbio1.princeton.edu/facility/flowcyt/...
    Added: Sat Feb 21 2009, Hits: 2613, Reviews: 0  Write review   Cached
  • Phycoerythrin Labeling (David Chambers)
    http://pingu.salk.edu/flow/protocols/pelabel.html
    Added: Tue May 14 2002, Hits: 1288, Reviews: 0  Write review   Cached
  • Protein (Antibody) Fluorescent Labeling (Mario Roederer)
    Gives detailed protocol for conjugation of proteins (principally monoclonal antibodies) to fluorescent dyes. The conjugations fall into four basic protocols: Type 1 (used for FITC, Cy5, and the initial preparation of the Cy5 and Cy7 tandem dyes); Type 2 (used for Biotin and Cascade Blue); Type 3 (used for conjugation of PE, APC, TR-BSA, and their derivatives); and Type 4 (used for the initial preparation of non-immunoglobulin proteins like Annexin V).
    http://www.drmr.com/abcon/
    Added: Tue May 14 2002, Hits: 2274, Reviews: 0  Write review   Cached
  • Staining Procedure for Flow Cytometric Detection of Human Cyclins (BD PharMingen)
    This is a standard protocol used at Pharmingen for Quality Control testing of the anti-cyclin antibodies by flow cytometry. It is a good starting point. However, investigators may need to optimize protocols for their own experimental system. It is particularly useful to refer to the published literature regarding protocols typically used for a given type of protein.
    http://www.bdbiosciences.com/pharmingen/protocols/...
    Added: Wed Aug 28 2002, Hits: 1654, Reviews: 0  Write review   Cached
  • Standard Flow Cytometry (FACS) Protocol (Jackson Lab)
    FACS Analysis Using Peripheral Blood Cells
    http://www.jax.org/imr/facs.html
    Added: Tue May 14 2002, Hits: 8449, Reviews: 0  Write review   Cached