Protocols
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Annexin V Staining Protocol
(BD PharMingen)
A general protocol for staining cell for cytometry analysis.
http://www.bdbiosciences.com/pharmingen/protocols/...
Added: Tue May 14 2002, Hits: 2586, Reviews: 0 Write review Cached -
Cytokines for FACS Analysis
(The Science Advisory Board)
Protocol for intracytoplasmic staining of cytokines for FACS analysis
http://www.scienceboard.net/resources/protocols.as...
Added: Mon Feb 23 2009, Hits: 3240, Reviews: 0 Write review Cached -
Flow Cytometric Analysis Of Bcl Family Members
(The Science Advisory Board)
Cell Fixation, staining and flow cytometric analysis
http://www.scienceboard.net/resources/protocols.as...
Added: Mon Feb 23 2009, Hits: 1270, Reviews: 0 Write review Cached -
Flow Cytometry Assay to measure Internalization of GPCR's
(Von Zastrow lab)
Internalization of receptor is measured as the reduction of receptors on the plasma membrane surface. Surface receptors are labeled with fluoresent antibodies which is in turn measured using a flow cytometer. The antibodies recognize an epitope engineered onto the N-terminus of the GPCR.
http://www.ucsf.edu/vonzast/flow.html
Added: Tue May 14 2002, Hits: 1487, Reviews: 0 Write review Cached -
Immunodetection of cyclin D1 and D2/D3 using flow cytometry
(The Science Advisory Board)
This protocol is for use with the D cyclins and employs 488 nm argon laser excitation of propidium iodide and 630 nm NeNe or diode laser excitation of the fluorochrome Cy5 to detect cell cycle-specific cyclin D expression. It has been tested with antibodies against cyclin D1, cyclin D2/D3 and cyclin E
http://www.scienceboard.net/resources/protocols.as...
Added: Mon Feb 23 2009, Hits: 1278, Reviews: 0 Write review Cached -
Intracellular Staining Protocol
(Flow Cytometry Core Facility, University of Chicago)
Staining of intracellular marker for FACS analysis
http://iacf.uchicago.edu/Protocols/IntracellularSt...
Added: Sat Feb 21 2009, Hits: 5461, Reviews: 0 Write review Cached