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Protocols
- IN-Gel Hybridization procedure for telomeric single stranded DNA detection (Wellinger Lab, Université de Sherbrooke )
Telomeric DNA is replicated by a special mechanism in which an enzyme called telomerase will elongate a short 3' overhang and create long, single stranded DNA at the ends of chromosomes. This enzyme requires a single stranded DNA portion as its substrate since the telomerase RNA moiety has to anneal with this DNA...
http://pages.usherbrooke.ca/wellinger/protocols.ht...
Added: Fri Jul 26 2002, Hits: 451, Reviews: 0 Write review Cached -
Protein-DNA Telomere FISH
(Stewart Lab, Washington University at St. Louis)
http://www.stewartlab.net/Protocols/Telomere_Fish_...
Added: Thu May 27 2004, Hits: 1055, Reviews: 0 Write review Cached -
Telomeric Repeat Amplification Protocol (TRAP)
(Stewart Lab, Washington University at St. Louis)
A method for assessing telomerase activity
http://www.stewartlab.net/Protocols/trap_assay_1.h...
Added: Thu May 27 2004, Hits: 558, Reviews: 0 Write review Cached -
Telomeric Repeat Amplification Protocol (TRAP) Telomerase Assay
(The Science Advisory Board)
This protocol describes a semi-quantitative in vitro assay to detect telomerase activity. Any active telomerase present in a cell extract from detergent-lysed cells will add a variable number of telomeric repeats (TTAGGG) to the 3' end of a substrate oligonucleotide. PCR that incorporates a radiolabeled nucleotide is then used to amplify the extension products. The presence of telomerase in the cell extracts is assessed by the appearance of a ladder of PCR products on a native polyacrylamide gel.
http://www.scienceboard.net/resources/protocols.as...
Added: Mon Feb 23 2009, Hits: 723, Reviews: 0 Write review Cached -
Terminal Restriction Fragment (TRF) Southern Blotting
(Stewart Lab, Washington University at St. Louis)
To determine telomeric restriction fragment (TRF) length.
http://www.stewartlab.net/Protocols/TRF_Southern_1...
Added: Thu May 27 2004, Hits: 629, Reviews: 0 Write review Cached