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Protocols
- Cell Migration, Chemotaxis and Invasion Assay (Corning)
Cell migration, the movement of cells from one area to another generally in response to a chemical signal, is central to achieving functions such as wound repair, cell differentiation, embryonic development and the metastasis of tumors. This protocol outlines the steps for conducting a cell invasion assay through a BME barrier.
http://www.selectscience.net/downloads/articles/14...
Added: Mon Feb 02 2009, Hits: 7692, Reviews: 0
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Matrigel Invasion Assays
(iProtocol)
Matirgel is considered as basement membrane and generated from EHS sarcoma. Invasion of tumor cells into Matrigel has been used to characterize involvement of ECM receptors and matrix degrading enzymes which play roles in tumor progression.
http://iprotocol.mit.edu/protocol/284.htm
Added: Mon Dec 09 2002, Hits: 538, Reviews: 0
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Cell Invasion Assay - 3D Growth on Collagen
(UAB)
http://homepage.uab.edu/wzhang/3dCellCultureOC.htm...
Added: Thu Jun 03 2010, Hits: 1038, Reviews: 0
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Cell Migration, Chemotaxis and Invasion Assay Protocol
(Corning)
This new 10-page protocol outlines the steps for conducting a Transwell insert-based cell invasion assay through a BME barrier with special notes for conducting a chemotaxis assay without a BME or ECM barrier. It is a generalized protocol and can be easily adapted to suit your needs.
http://catalog2.corning.com/Lifesciences/media/pdf...
Added: Wed Feb 11 2009, Hits: 5831, Reviews: 0
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Invasion Assay
(Bissell Lab, Berkely Lab)
http://www.lbl.gov/LBL-Programs/lifesciences/Bisse...
Added: Mon Feb 02 2009, Hits: 4652, Reviews: 0
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Matrigel Invasion Assay
(The Science Advisory Board)
Matirgel is considered as basement membrane and generated from EHS sarcoma. Matrigel contains not only basement membrane components (collagens, laminin, and proteoglycans)but also matrix degrading enzymes/their inhibitors and growth factors. Invasion of tumor cells into Matrigel has been used to characterize involvement of ECM receptors and matrix degrading enzymes which play roles in tumor progression.
http://www.scienceboard.net/resources/protocols.as...
Added: Wed Apr 21 2004, Hits: 4319, Reviews: 0
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Metrigel Invasion Assay
(UAB)
http://homepage.uab.edu/wzhang/MatrigelInvasionAss...
Added: Thu Jun 03 2010, Hits: 958, Reviews: 0
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