Summary: The Boyden chamber transwell assay, originally introduced by Boyden for the analysis of leukocyte chemotaxis, is based on a chamber of two medium-filled compartments separated by a microporous membrane. The Boyden chamber is a useful tool to study cell migration and cell invasion. In general, cells are placed in the upper compartment and are allowed to migrate through the pores of the membrane into the lower compartment, in which chemotactic agents are present. After an appropriate incubation time, the membrane between the two compartments is fixed and stained, and the number of cells that have migrated to the lower side of the membrane is determined. Therefore, the Boyden chamber-based cell migration assay has also been called filter membrane migration assay, trans-well migration assay, or chemotaxis assay.
Protocols
- Transwell (Boyden Chamber) Cell Migration Assay (University of Chicago Molecular Oncology Laboratory)
http://www.boneandcancer.org/protocolse16.htm
Added: Thu Jun 03 2010, Hits: 5676, Reviews: 0 Write review - Transwell Filter Migration Assay
(Graingerlab,Department of Medicine, University of Cambridge)
The transwell filter migration assay is a relatively simple cell migration assay. It is suitable for the screening of compounds to determine their chemoattractant or inhibitory effects on cell migration, and provides a rapid indication of net cell population movement responses to putative chemoattractants or cell migration inhibitors. A variety of motile cell types (including freshly isolated and cultured leukocytes) can be used in this system.
http://www.graingerlab.org/assets/grl01/protocols/...
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Boyden Chamber Assay
(Chen HC, Methods in Molecular Biology, vol. 294: Cell Migration: Developmental Methods and Protocols)
Detailed protocol for measuring the migration of Madin-Darby canine kidney cells using a 48-well chamber.
http://www.protocol-online.org/protocols/4464.pdf
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Transwell Migration Assay
(Nature Protocol)
http://www.natureprotocols.com/2006/08/07/transwel...
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Transwell Migration Assay
(Shimizu Lab, University of Minnesota)
http://www.tc.umn.edu/~shimi002/SOPtranswell.pdf
Added: Thu Jun 03 2010, Hits: 3441, Reviews: 0 Write review