WB for Membrane Protein - Not able to see membrane protein on western (Aug/03/2005 )
Hi Prajwal
Thanks so much for the wise suggestion! i can definetely try that out!
I am in India and am working/studying in India too...
Adithi.
-molbionovice-
Don't boil your sample before loading it on a gel and see what happens.
I work with 3 membrane bound proteins and none of them will show up on western if I boil them. Just let them sit at room temp for 30 min suspended in the protein solublilization buffer.
-pBluescript-
QUOTE (pBluescript @ Aug 11 2005, 01:14 AM)
Don't boil your sample before loading it on a gel and see what happens.
I work with 3 membrane bound proteins and none of them will show up on western if I boil them. Just let them sit at room temp for 30 min suspended in the protein solublilization buffer.
I work with 3 membrane bound proteins and none of them will show up on western if I boil them. Just let them sit at room temp for 30 min suspended in the protein solublilization buffer.
Do you increase the quantity of SDS to denature the sample insted?
-Rosie-