plant protein purification - pectin removal from crude extract (Jul/03/2005 )

Hi,

I am extracting a protein from plant system, from a fruit that contains good amount of pectic material.The protein has good activity in crude extract for as many days , but looses activity upon subjecting to any type of column chromatograhy.Also I am not able to separate the pectic substances from my protein .Kindly help me by giving sugestions how to circumvent the problem.

Thank-you.

Hi,

I am extracting a protein from plant system, from a fruit that contains good amount of pectic material.The protein has good activity in crude extract for as many days , but looses activity upon subjecting to any type of column chromatograhy.Also I am not able to separate the pectic substances from my protein .Kindly help me by giving sugestions how to circumvent the problem.

Thank-you.