MBP tagged recombinant protein - (Apr/15/2005 )
does anyone have purified MBP tagged recombinant protein? any tips?
I am going to do this, try to make the protein soluble rather than GST tagged which was tried before the protein is not soluble.
cathy
-cathy-
However, it is very likely after cleaving the MBP tag, the protein will precipitate out.
It really depends on the property of your protein.
Think about both flexible and rigid(helix) links too.
QUOTE (cathy @ Apr 15 2005, 03:09 AM)
does anyone have purified MBP tagged recombinant protein? any tips?
I am going to do this, try to make the protein soluble rather than GST tagged which was tried before the protein is not soluble.
cathy
I am going to do this, try to make the protein soluble rather than GST tagged which was tried before the protein is not soluble.
cathy
-GoGoalGirl-