interpretation of the MALDI data................... - How to understand it............... (Dec/27/2008 )
kindly help me to understand this................
Taxonomy : Homo sapiens (human) (147916 sequences)
Timestamp : 13 Aug 2008 at 12:03:45 GMT
Protein hits : Q9UQ35_HUMAN RNA binding protein.- Homo sapiens (Human). BAA31657 AB014582 NID: - Homo sapiens Q9Y632_HUMAN APC2 protein (Fragment).- Homo sapiens (Human). O95996_HUMAN APCL protein.- Homo sapiens (Human). AAB41132 HSU38291 NID: - Homo sapiens
Probability Based Mowse Score
Ions score is -10*Log(P), where P is the probability that the observed match is a random event.
Individual ions scores > 42 indicate identity or extensive homology (p<0.05).
Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein hits.
Peptide Summary Report
Protein Summary (deprecated) Peptide Summary Select Summary (protein hits) Select Summary (unassigned) Export Search Results Help
Significance threshold p< Max. number of hits
Standard scoring MudPIT scoring Ions score or expect cut-off Show sub-sets
Show pop-ups Suppress pop-ups Sort unassigned Decreasing Score Increasing query / Mr Decreasing Intensity Require bold red
Error tolerant
1. Q9UQ35_HUMAN Mass: 300239 Score: 63 Queries matched: 5
RNA binding protein.- Homo sapiens (Human).
Check to include this hit in error tolerant search or archive report
Query Observed Mr(expt) Mr(calc) Delta Miss Score Expect Rank Peptide
220 502.6686 1003.3227 1002.5206 0.8021 1 22 7 1 R.SRSGSSPGLR.D
255 512.6286 1023.2427 1022.4702 0.7725 1 10 85 3 R.SNSSPEMKK.S + Oxidation (M)
342 612.7286 1223.4427 1224.5847 -1.1420 0 23 4.1 1 R.TSVPENHAQSR.I
377 460.2282 1377.6627 1377.7365 -0.0737 0 13 39 6 R.TPQAPASANLVGPR.S
421 522.6215 1564.8427 1563.7199 1.1229 1 5 2.2e+002 8 R.SSSASSPEMKDGLPR.T + Oxidation (M)
Proteins matching the same set of peptides:
O15038_HUMAN Mass: 304986 Score: 63 Queries matched: 5
KIAA0324 protein (Fragment).- Homo sapiens (Human).
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2. BAA31657 Mass: 109177 Score: 50 Queries matched: 4
AB014582 NID: - Homo sapiens
Check to include this hit in error tolerant search or archive report
Query Observed Mr(expt) Mr(calc) Delta Miss Score Expect Rank Peptide
18 318.9486 635.8827 635.3391 0.5436 0 6 2.3e+002 5 R.FGSLGR.V
190 325.0149 972.0227 973.4981 -1.4754 0 15 35 5 K.SFGDPAKPR.A
299 360.6415 1078.9027 1077.4808 1.4219 1 10 1e+002 2 K.QRGPCAMSR.L + Oxidation (M)
387 467.9349 1400.7827 1401.6810 -0.8982 1 19 9 7 K.KLQDTPSEPMEK.D
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3. Q9Y632_HUMAN Mass: 133573 Score: 47 Queries matched: 5
APC2 protein (Fragment).- Homo sapiens (Human).
Check to include this hit in error tolerant search or archive report
Query Observed Mr(expt) Mr(calc) Delta Miss Score Expect Rank Peptide
98 358.4686 714.9227 714.3231 0.5996 0 11 1.1e+002 6 R.QAMGHR.H + Oxidation (M)
185 483.9236 965.8327 964.3855 1.4472 0 11 73 6 R.AQSCSPCR.G
230 340.5715 1018.6927 1018.4931 0.1996 0 (14) 47 4 K.DTSGEAAVAAK.A
248 340.9882 1019.9427 1018.4931 1.4496 0 19 12 1 K.DTSGEAAVAAK.A
374 685.9386 1369.8627 1371.7115 -1.8487 1 11 63 4 K.HKMIAMGSAAALR.N + Oxidation (M)
Proteins matching the same set of peptides:
Q9UEM8_HUMAN Mass: 176917 Score: 43 Queries matched: 5
APC2 protein (Fragment).- Homo sapiens (Human).
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4. O95996_HUMAN Mass: 245966 Score: 44 Queries matched: 6
APCL protein.- Homo sapiens (Human).
Check to include this hit in error tolerant search or archive report
Query Observed Mr(expt) Mr(calc) Delta Miss Score Expect Rank Peptide
24 325.0736 648.1327 647.3173 0.8154 1 3 6.7e+002 3 K.DARMR.A
98 358.4686 714.9227 714.3231 0.5996 0 11 1.1e+002 6 R.QAMGHR.H + Oxidation (M)
185 483.9236 965.8327 964.3855 1.4472 0 11 73 6 R.AQSCSPCR.G
230 340.5715 1018.6927 1018.4931 0.1996 0 (14) 47 4 K.DTSGEAAVAAK.A
248 340.9882 1019.9427 1018.4931 1.4496 0 19 12 1 K.DTSGEAAVAAK.A
374 685.9386 1369.8627 1371.7115 -1.8487 1 11 63 4 K.HKMIAMGSAAALR.N + Oxidation (M)
--------------------------------------------------------------------------------
5. AAB41132 Score: 43 Queries matched: 6
HSU38291 NID: - Homo sapiens
Check to include this hit in error tolerant search or archive report
Query Observed Mr(expt) Mr(calc) Delta Miss Score Expect Rank Peptide
78 342.6886 683.3627 684.4646 -1.1019 1 17 15 2 R.LVLRGK.R
81 342.7186 683.4227 684.4646 -1.0419 1 (5) 2.6e+002 5 R.LVLRGK.R
83 342.7986 683.5827 682.4490 1.1337 1 17 21 1 R.LPLRGK.R
143 433.7636 865.5127 867.4199 -1.9071 0 12 53 8 K.HQAQEQK.D
391 484.6082 1450.8027 1450.6472 0.1555 1 9 96 8 K.DSKEMQFLMYK.W + 2 Oxidation (M)
411 512.4615 1534.3627 1533.7271 0.6357 1 16 21 3 R.SRSDTSQVTPAEEK.D
--------------------------------------------------------------------------------
Peptide matches not assigned to protein hits: (no details means no match)
Query Observed Mr(expt) Mr(calc) Delta Miss Score Expect Rank Peptide
48 669.1900 668.1827 668.3897 -0.2070 0 28 1.1 1 FVYLK
369 448.8815 1343.6227 1344.6456 -1.0229 0 28 1.4 1 GTEGHSVMLATAR + Oxidation (M)
284 523.8436 1045.6727 1045.5291 0.1436 0 27 1.9 1 LSSPADITDK
19 320.9636 639.9127 640.2890 -0.3763 0 27 1.3 1 MATTAF
395 493.9782 1478.9127 1478.6922 0.2205 0 26 1.9 1 EEELSLQDITMR + Oxidation (M)
357 433.7649 1298.2727 1296.6245 1.6483 1 26 2.1 1 SDRIVTCGAYR
294 358.3382 1071.9927 1070.5583 1.4344 0 25 3.1 1 FMQIITFR + Oxidation (M)
333 405.4882 1213.4427 1214.7095 -1.2668 1 25 2.6 1 ASGRVLSLSGIR
110 386.8286 771.6427 772.4443 -0.8016 0 25 4.1 1 LLSLGDR
201 327.5849 979.7327 979.5815 0.1513 0 25 3.2 1 VTQLPGPIR
171 310.6082 928.8027 929.5545 -0.7518 1 24 3.7 1 LSALAEAKK
411 512.4615 1534.3627 1532.7484 1.6143 1 24 2.9 1 YLEAHFGGADAARR
268 342.8049 1025.3927 1024.4607 0.9320 1 24 3.1 1 MSAAKGSDSR + Oxidation (M)
398 745.3686 1488.7227 1489.7698 -1.0471 0 24 2.9 1 LTIESMPLSVAEGK + Oxidation (M)
272 342.8515 1025.5327 1026.5168 -0.9841 1 24 3.2 1 YMNSQKLK + Oxidation (M)
387 467.9349 1400.7827 1400.7776 0.0051 1 24 3.2 1 AGLKDAVVTQFPR
240 340.7215 1019.1427 1018.4931 0.6496 0 24 4.7 1 DPGSLSQTSK
292 358.1015 1071.2827 1069.5516 1.7311 1 23 4.7 1 QREPADGLGK
389 476.6749 1427.0027 1428.7296 -1.7269 1 23 3.7 1 FQRLCGVEAPPR
20 322.5786 643.1427 643.3905 -0.2477 0 23 6.1 1 SPISLK
296 358.4715 1072.3927 1071.4576 0.9351 0 23 5.3 1 MDMGTSALSK + 2 Oxidation (M)
252 341.8515 1022.5327 1021.5305 1.0023 1 23 4.7 1 RESPPPSPR
364 436.3115 1305.9127 1306.7319 -0.8192 0 23 4.5 1 MVIEYIGTIIR
130 414.8686 827.7227 829.4658 -1.7430 0 23 6.2 1 AGESVVLR
227 340.5182 1018.5327 1017.5091 1.0237 1 22 6.2 1 QSSEPSKGAK
182 318.9515 953.8327 955.5087 -1.6759 1 22 5 1 EENPALRK
108 385.9786 769.9427 769.3970 0.5457 0 22 5.5 1 APTPQEK
328 398.4982 1192.4727 1193.6557 -1.1830 0 22 6 1 DLHWGNVLLK
432 544.8115 1631.4127 1630.9267 0.4860 1 22 4.7 1 AKNASGRPLPLGPPTR
61 340.7236 679.4327 680.3493 -0.9166 0 22 5.6 1 EPPPNK
137 420.6586 839.3027 840.3800 -1.0773 0 22 5.8 1 QMFGGGTK + Oxidation (M)
361 434.0649 1299.1727 1300.5830 -1.4103 1 22 5.5 1 IGETAPRSSSHC
214 330.9282 989.7627 991.5311 -1.7684 1 21 7.6 1 APKQQQHR
76 342.6636 683.3127 684.3555 -1.0428 0 21 6.2 1 SNVTHK
287 529.7386 1057.4627 1057.4611 0.0017 0 21 8.3 1 EHNENMLR + Oxidation (M)
262 342.6882 1025.0427 1023.5502 1.4926 1 21 6.9 1 FARAFSTPK
400 502.6682 1504.9827 1506.8419 -1.8592 1 21 6.1 1 AFIHSSALIKHQR
191 325.0749 972.2027 970.5923 1.6104 1 21 8.9 1 ETLRLAIR
190 325.0149 972.0227 970.5420 1.4807 1 21 9 1 RPSRVSNR
145 433.9536 865.8927 867.4450 -1.5523 1 20 7.9 1 FQSSKSGK
213 495.7536 989.4927 988.4661 1.0266 0 20 9.9 1 MRPYNHR + Oxidation (M)
365 442.4682 1324.3827 1322.7419 1.6408 1 20 7.7 1 LSPLLPGGGGRGSR
322 388.3582 1162.0527 1161.6618 0.3909 0 20 8.6 1 TLHAALGGLPGR
311 375.0349 1122.0827 1123.5253 -1.4426 0 20 8.6 1 LLDCSMEIK + Oxidation (M)
119 391.1886 780.3627 780.4204 -0.0577 0 20 9 1 FTCLLK
390 483.9249 1448.7527 1450.7234 -1.9706 0 20 7.6 1 TLMLLAQMSMISP + Oxidation (M)
78 342.6886 683.3627 684.4282 -1.0655 1 20 7.8 1 ARLTPK
396 494.4015 1480.1827 1481.6872 -1.5045 1 20 7.1 1 EHQFSHAGRSNGR
313 383.7182 1148.1327 1149.5414 -1.4087 0 20 9.4 1 LENVSFGDNR
330 400.2315 1197.6727 1197.5924 0.0803 1 20 9.2 1 APEPAARAMER
323 388.5949 1162.7627 1164.5815 -1.8188 1 20 9.7 1 EDKAFLWEK
217 331.8882 992.6427 992.5039 0.1388 0 20 11 1 SIGVSNFNR
174 311.6082 931.8027 930.4923 1.3104 1 20 12 1 WRELAEK
347 414.8682 1241.5827 1239.6104 1.9723 1 19 9.4 1 CARMYAQLVI + Oxidation (M)
338 610.0836 1218.1527 1219.7764 -1.6237 1 19 11 1 LVAASRHLILK
225 508.6386 1015.2627 1013.5692 1.6936 0 19 12 1 LPGGMLLASR
125 405.4886 808.9627 809.3767 -0.4139 1 19 10 1 SKESVSSS
242 340.7882 1019.3427 1017.5719 1.7708 1 19 13 1 RAEVPPPPR
355 428.7915 1283.3527 1283.6106 -0.2579 0 19 10 1 DGQNGVPGLDGQK
178 312.9649 935.8727 934.4542 1.4185 1 19 12 1 IREMDQK + Oxidation (M)
195 326.5682 976.6827 978.5498 -1.8671 1 19 13 1 NKTLSAAFK
200 327.5149 979.5227 980.3981 -0.8754 0 19 11 1 NMSSAGADGR + Oxidation (M)
343 411.1482 1230.4227 1229.6364 0.7863 1 19 11 1 IPESSNVGGSRK
175 311.7815 932.3227 932.4424 -0.1197 1 19 14 1 SSGGGGGGGGKR
394 492.5615 1474.6627 1475.8460 -1.1833 1 19 10 1 HGPISEVLLIKDR
129 414.8186 827.6227 826.5388 1.0839 1 19 15 1 ALLVAKGR
77 342.6736 683.3327 683.2796 0.0531 0 19 11 1 STAAMST + Oxidation (M)
192 325.2215 972.6427 971.5036 1.1391 0 19 14 1 ELAEAVAGGR
27 326.5686 651.1227 649.3184 1.8044 0 19 13 1 SYGAPR
426 529.8849 1586.6327 1586.7915 -0.1588 1 19 10 1 WYAITFAMRLDGK + Oxidation (M)
244 340.8515 1019.5327 1017.5719 1.9608 0 19 14 1 ARPPAQGPPK
350 418.7215 1253.1427 1251.6434 1.4993 1 19 11 1 QWMKVQAAFK + Oxidation (M)
259 342.6482 1024.9227 1024.5818 0.3409 0 19 12 1 WPGILVGGAR
431 544.0749 1629.2027 1630.9307 -1.7280 1 18 11 1 KLLPSQNGFHHILK
33 327.5836 653.1527 651.4068 1.7459 0 18 10 1 IPGIPR
271 342.8315 1025.4727 1024.5203 0.9525 1 18 12 1 GHRWDQVK
392 485.4282 1453.2627 1452.5525 0.7102 0 18 11 1 XXAMINGPMPWNT
327 391.1882 1170.5427 1170.6859 -0.1432 0 18 13 1 SNELLELILK
149 435.7186 869.4227 870.5650 -1.1423 1 18 13 1 KASLALIR
316 386.8282 1157.4627 1157.6153 -0.1525 1 18 14 1 GLQELTDARR
197 327.3115 978.9127 978.4957 0.4170 0 18 14 1 MAFPGVSVR + Oxidation (M)
176 467.9336 933.8527 934.4542 -0.6015 1 18 16 1 AAVKDSCGK
298 360.5549 1078.6427 1078.5151 0.1276 0 18 14 1 MPVGTQATMK + Oxidation (M)
386 465.8549 1394.5427 1395.6452 -1.1025 1 18 12 1 YLAEVACGDDRK
251 512.1186 1022.2227 1020.4910 1.7317 0 18 14 1 GGSMIDANLK + Oxidation (M)
97 358.3386 714.6627 716.3534 -1.6906 0 18 20 1 FFEFK
429 533.8249 1598.4527 1597.8464 0.6063 1 18 11 1 IGEAKNLPTYPGPNK
383 464.7249 1391.1527 1390.6762 0.4765 1 18 12 1 AKADMTIVDNEGK
212 330.5015 988.4827 989.5083 -1.0256 0 18 18 1 VSSWLWGR
406 508.6382 1522.8927 1521.7358 1.1569 1 18 13 1 YVCQAKNDAGIQR
246 340.9315 1019.7727 1018.5481 1.2246 0 18 17 1 QLAGGMVSLK + Oxidation (M)
55 340.6386 679.2627 680.4836 -1.2209 0 18 17 1 ILPLLL
154 444.1536 886.2927 884.4136 1.8792 0 18 19 1 IMFSEMK
455 701.6449 2101.9127 2102.0354 -0.1227 1 18 9.1 1 DAKNLIPMDPNGLSDPYVK + Oxidation (M)
165 460.4386 918.8627 918.5135 0.3493 0 18 18 1 SVGVVTTTR
345 412.6549 1234.9427 1234.6591 0.2836 1 18 15 1 MAGISSIDAVKK + Oxidation (M)
205 327.7349 980.1827 978.5471 1.6356 1 18 16 1 HRNGGLLGR
332 403.6149 1207.8227 1206.5670 1.2558 0 18 15 1 GEPGPPGPAGFPGA
360 434.0515 1299.1327 1299.6857 -0.5529 0 18 14 1 MSATLILEPPGR + Oxidation (M)
289 530.1086 1058.2027 1056.6291 1.5736 1 18 19 1 IVLDVNKTR
10 310.6086 619.2027 620.3129 -1.1102 0 18 23 1 NVSSSK
122 400.2286 798.4427 798.3984 0.0443 0 18 16 1 TPSPAGGGR
319 387.8215 1160.4427 1158.5452 1.8976 0 18 17 1 AVIHMGSGESR + Oxidation (M)
81 342.7186 683.4227 684.3490 -0.9262 1 17 16 1 MGHRGK
337 610.0136 1218.0127 1216.4763 1.5364 0 17 17 1 LLQTGQXXAXPG
443 609.9949 1826.9627 1825.7175 1.2452 0 17 11 1 CGYFFLDDTDPDSMK + Oxidation (M)
349 418.6715 1252.9927 1254.7085 -1.7157 0 17 16 1 QVVNPPSFIVR
243 340.8415 1019.5027 1018.4931 1.0096 1 17 20 1 EEAQEKTGK
241 340.7715 1019.2927 1020.4360 -1.1433 0 17 21 1 ENGGSSDDIK
74 342.3036 682.5927 681.3558 1.2369 0 17 17 1 AAVEHR
236 340.6749 1019.0027 1017.5090 1.4937 1 17 21 1 EIKASEGER
228 340.5549 1018.6427 1019.5036 -0.8609 0 17 21 1 AEGVPASAYR
307 553.0336 1104.0527 1104.5485 -0.4958 0 17 20 1 GGMETLELQK
368 444.1549 1329.4427 1328.7023 0.7404 1 17 17 1 MPGIAGRAVAGWK + Oxidation (M)
141 428.7936 855.5727 856.4767 -0.9039 1 17 20 1 GADNLVRI
440 581.6182 1741.8327 1741.8258 0.0069 0 17 13 1 LDEGSDVESEPDLPLK
203 327.6249 979.8527 978.5862 1.2665 0 17 19 1 VPPDAVLLR
66 340.9186 679.8227 680.3493 -0.5266 0 17 18 1 EPPPNK
193 325.3782 973.1127 971.3953 1.7174 0 17 22 1 FGSGMNMGR + Oxidation (M)
267 342.7982 1025.3727 1023.4985 1.8742 0 17 18 1 EAATDPPAPR
454 685.9382 2054.7927 2053.8905 0.9022 1 17 11 1 MDNISRAAGNTSESQSCAR
356 431.7882 1292.3427 1291.5728 0.7700 0 17 18 1 MEPGAGHLDGHR + Oxidation (M)
196 327.0782 978.2127 976.4879 1.7248 0 17 21 1 GHFLYQGR
245 340.9215 1019.7427 1018.5659 1.1769 0 17 21 1 LNLSVTSSAK
367 442.7015 1325.0827 1324.7252 0.3575 0 17 18 1 HTLAPNVVAFTR
184 322.5749 964.7027 965.4930 -0.7903 1 17 21 1 YNKGLTDR
253 512.4586 1022.9027 1021.5127 1.3900 0 17 19 1 HQIVHMGGK + Oxidation (M)
207 492.5636 983.1127 984.5240 -1.4113 1 17 19 1 DLPQEKQK
198 327.4349 979.2827 977.4852 1.7976 0 17 20 1 EGASVQIMK + Oxidation (M)
270 342.8149 1025.4227 1026.4474 -1.0247 0 17 18 1 CASGTMGSIK + Oxidation (M)
143 433.7636 865.5127 866.4974 -0.9847 0 17 19 1 VTHIEIR
331 403.3349 1206.9827 1206.6945 0.2882 0 17 19 1 RPLQGDRPLR
370 448.8882 1343.6427 1343.6139 0.0288 0 17 18 1 AMPSPQTEAAEGR
273 342.9582 1025.8527 1027.5047 -1.6519 0 17 19 1 LGHGNSSTQK
53 340.6286 679.2427 680.2435 -1.0008 0 17 22 1 GGNESAM + Oxidation (M)
352 420.4449 1258.3127 1259.6734 -1.3607 0 17 19 1 HPAGLAAAAAGTPR
172 465.8536 929.6927 930.4923 -0.7996 1 16 24 1 REALWEK
142 431.7886 861.5627 861.4192 0.1435 0 16 27 1 TASTETPR
379 460.2449 1377.7127 1378.7279 -1.0151 1 16 19 1 SKSLAFHTMLTK + Oxidation (M)
265 342.7182 1025.1327 1026.4263 -1.2935 0 16 19 1 DMAGYMPAR + Oxidation (M)
162 460.2286 918.4427 916.4866 1.9562 0 16 26 1 SLSPGLDTK
269 342.8115 1025.4127 1024.4430 0.9697 1 16 19 1 DKNCVMSR + Oxidation (M)
79 342.6936 683.3727 683.4218 -0.0490 0 16 18 1 VPEVIK
107 383.7186 765.4227 765.3691 0.0537 1 16 21 1 AMSAKDK + Oxidation (M)
408 510.3949 1528.1627 1528.7960 -0.6332 1 16 17 1 GFFLVQTKEVSMK + Oxidation (M)
409 511.6915 1532.0527 1531.7300 0.3227 1 16 18 1 MELDGQKGQELER
415 520.5549 1558.6427 1559.7000 -1.0572 0 16 17 1 MYELSCYLPQEK
448 610.9049 1829.6927 1829.9492 -0.2564 1 16 15 1 TVVMQGAAGIGKSMLAHK + 2 Oxidation (M)
96 358.3286 714.6427 716.3341 -1.6914 0 16 31 1 DSGVPDK
229 340.5615 1018.6627 1019.5434 -0.8806 1 16 25 1 QLTMVEKR + Oxidation (M)
75 342.6486 683.2827 684.4282 -1.1455 0 16 20 1 ILVANR
366 442.6849 1325.0327 1324.6809 0.3518 1 16 20 1 RISGTSIAFMNI + Oxidation (M)
88 342.8486 683.6827 682.3108 1.3719 0 16 23 1 QFMNK + Oxidation (M)
261 342.6749 1025.0027 1026.4804 -1.4777 0 16 21 1 MPPSPLDDR
204 327.6649 979.9727 979.4757 0.4970 0 16 23 1 ITISCSGSR
438 563.8882 1688.6427 1688.8039 -0.1612 0 16 18 1 TEITTNCLSDHSAIK
158 449.0236 896.0327 896.4240 -0.3912 0 16 22 1 STYGGTAALG
351 420.2815 1257.8227 1256.6989 1.1238 1 16 22 1 RDFIILGNGPR
26 325.3786 648.7427 649.2676 -0.5249 0 16 33 1 MCPAR + Oxidation (M)
14 312.3336 622.6527 622.3439 0.3089 0 16 30 1 VYSVR
104 360.8486 719.6827 720.3766 -0.6939 0 16 30 1 LSTGTSR
126 411.1486 820.2827 819.4524 0.8303 0 16 29 1 LSLISCK
263 342.6949 1025.0627 1023.4365 1.6262 0 16 22 1 MLGADVDCK + Oxidation (M)
177 312.3349 933.9827 932.4386 1.5442 0 16 28 1 CGGLAAGAEK
216 331.3582 991.0527 990.4665 0.5862 1 16 29 1 RAAGGGGGGGMK + Oxidation (M)
403 503.0915 1506.2527 1504.5931 1.6596 1 16 21 1 CEFCDVRCTMK
317 387.0215 1158.0427 1159.5655 -1.5228 1 16 26 1 RQEMDPGLSK
202 327.6015 979.7827 978.5056 1.2771 0 16 25 1 GSTVVELMK + Oxidation (M)
235 340.6515 1018.9327 1018.5481 0.3846 0 16 28 1 QLAGGMVSLK + Oxidation (M)
232 340.6282 1018.8627 1018.5593 0.3034 1 16 29 1 LRSNLMGTK
166 464.6836 927.3527 929.3259 -1.9732 0 16 25 1 DDDFMCK
199 327.4815 979.4227 979.4141 0.0086 0 16 26 1 MGSNSGQAGR + Oxidation (M)
354 420.6615 1258.9627 1257.5765 1.3862 0 16 24 1 EEYLLFDSDK
135 420.4486 838.8827 839.3886 -0.5058 0 16 24 1 HQGAGENK
266 342.7449 1025.2127 1024.5012 0.7116 0 16 23 1 APTVHEMPK + Oxidation (M)
353 420.5882 1258.7427 1259.6292 -0.8865 0 16 24 1 QVVQATAEQMR
430 542.5682 1624.6827 1625.7680 -1.0853 1 16 20 1 MEEKYLPELMAEK + Oxidation (M)
399 500.9449 1499.8127 1501.6579 -1.8452 0 15 22 1 NSNSPPSPSSMNQR
183 320.9649 959.8727 961.5192 -1.6465 0 15 30 1 SGTSASLAIR
444 610.0149 1827.0227 1826.8323 0.1905 0 15 17 1 QFPDDVEDAPDLNAPGK
378 460.2449 1377.7127 1379.7045 -1.9918 1 15 23 1 EGTKGDPGPAGLPGK
39 329.4086 656.8027 656.2952 0.5075 0 15 33 1 EPHMK + Oxidation (M)
238 340.7049 1019.0927 1019.4205 -0.3277 0 15 30 1 SFANFMCK + Oxidation (M)
425 529.7382 1586.1927 1584.6912 1.5015 1 15 21 1 LQEECAFREEMK + Oxidation (M)
336 609.9936 1217.9727 1216.4763 1.4964 0 15 27 1 LLQTGQXXAXPG
40 330.2186 658.4227 660.3443 -1.9215 0 15 45 1 AADVTGK
180 314.7815 941.3227 942.5498 -1.2271 1 15 26 1 ALKLGQEGK
71 341.8486 681.6827 681.3082 0.3745 0 15 22 1 EGHDPK
321 581.6186 1161.2227 1160.6917 0.5310 0 15 29 1 AYLQVLSVLR
279 520.7186 1039.4227 1037.4964 1.9263 0 15 26 1 LADFGSCLR
377 460.2282 1377.6627 1378.6398 -0.9771 1 15 25 1 EDPEMSTALSRK + Oxidation (M)
435 553.4449 1657.3127 1658.9389 -1.6262 1 15 21 1 NVGLQLATLLMSQKK + Oxidation (M)
295 358.4682 1072.3827 1070.5906 1.7921 1 15 32 1 QKPMAKGPAK + Oxidation (M)
428 533.7482 1598.2227 1596.7467 1.4760 1 15 21 1 GSMKTHFNTAQGFR + Oxidation (M)
407 510.1482 1527.4227 1527.8158 -0.3930 1 15 22 1 IERFNPPSNVTVR
254 342.0582 1023.1527 1024.5414 -1.3887 1 15 27 1 GSKGAPGPAGAR
151 442.4686 882.9227 882.3728 0.5500 0 15 28 1 MNSSWMK
134 420.2836 838.5527 839.3266 -0.7738 0 15 27 1 TMNGDMR + Oxidation (M)
185 483.9236 965.8327 966.5022 -0.6694 1 15 30 1 AKSYIEEK
211 330.2182 987.6327 988.4826 -0.8498 0 15 35 1 VASPGGDTTGK
219 500.9436 999.8727 999.4709 0.4019 0 15 32 1 TNGMHWVR
247 340.9582 1019.8527 1018.5131 1.3397 1 15 32 1 RWQGVSMR
226 510.1486 1018.2827 1017.4549 0.8278 0 15 35 1 AICSDPNNK
222 503.0886 1004.1627 1002.4982 1.6645 0 15 33 1 NIPSNATSVT
106 375.0336 748.0527 749.3555 -1.3028 0 15 41 1 SSASSSPK
310 558.8886 1115.7627 1116.6907 -0.9279 0 15 30 1 LFSNLILIGK
414 512.9415 1535.8027 1533.8263 1.9764 1 15 24 1 RVGYLTGLPTTNSR
116 388.6136 775.2127 776.4657 -1.2530 1 15 42 1 VRIFSR
189 324.9915 971.9527 973.4287 -1.4760 0 15 34 1 NNSLHDMK + Oxidation (M)
18 318.9486 635.8827 635.2948 0.5879 0 15 33 1 AMSALQ + Oxidation (M)
373 451.1882 1350.5427 1350.6429 -0.1002 0 15 34 1 FSHSHSQTPAPR
144 433.7786 865.5427 864.4705 1.0722 1 15 29 1 AFDLSGKK
63 340.7886 679.5627 681.4425 -1.8798 0 15 30 1 LNPLLI
95 358.1036 714.1927 715.3977 -1.2049 1 15 37 1 RANLDK
136 420.5886 839.1627 838.4436 0.7191 0 15 29 1 EVSTYIK
112 387.6436 773.2727 774.4024 -1.1297 0 15 45 1 GHYTIGK
305 365.4515 1093.3327 1092.4982 0.8345 1 15 31 1 NMSSAGADGRK
86 342.8136 683.6127 685.3983 -1.7856 1 15 33 1 GRAGAVR
264 342.7115 1025.1127 1024.5706 0.5422 1 15 29 1 LKGFFQSAK
301 360.8515 1079.5327 1078.5441 0.9886 0 15 30 1 ATNMTVSAIR + Oxidation (M)
223 503.0936 1004.1727 1003.4974 0.6753 0 15 35 1 GLPFEAENK
147 434.0636 866.1127 867.4814 -1.3687 0 15 31 1 VHLGSVEK
160 459.1436 916.2727 914.5007 1.7720 1 15 39 1 SKLMAPPR + Oxidation (M)
230 340.5715 1018.6927 1019.4818 -0.7891 1 15 37 1 EQVGRMER + Oxidation (M)
339 610.7636 1219.5127 1217.6377 1.8750 1 15 33 1 GQGAYRPGTRR
382 464.7049 1391.0927 1391.8024 -0.7097 1 14 28 1 LAVFTKATLTTVQ
445 610.0849 1827.2327 1826.8620 0.3707 1 14 22 1 EGPAGGTGGSGGPGGSLGSRGR
159 451.1886 900.3627 901.4981 -1.1354 1 14 38 1 QRSSPVTK
344 412.5115 1234.5127 1233.5369 0.9758 1 14 32 1 LDCKSSMYSK + Oxidation (M)
275 346.6849 1037.0327 1037.6597 -0.6270 1 14 32 1 IQAKLPGIAK
237 340.6915 1019.0527 1020.6444 -1.5917 0 14 38 1 ILHILGSIR
439 581.5015 1741.4827 1740.9411 0.5417 0 14 24 1 TSGTIIFGGGTYVTVLR
348 415.0482 1242.1227 1242.6080 -0.4853 1 14 32 1 MRGWPAPAWR + Oxidation (M)
334 609.9086 1217.8027 1219.7764 -1.9737 1 14 34 1 LVAASRHLILK
434 553.0349 1656.0827 1656.7772 -0.6945 0 14 24 1 GSMLALAMVSLEMEK + 3 Oxidation (M)
404 504.0815 1509.2227 1508.6777 0.5451 0 14 30 1 MSEQTAALDTSSPR + Oxidation (M)
29 327.3136 652.6127 653.3245 -0.7118 0 14 27 1 GPGPGGGR
258 342.3049 1023.8927 1022.5648 1.3280 1 14 32 1 LEELYTKK
90 685.2300 684.2227 684.3918 -0.1691 0 14 36 1 LAAAPSR
308 553.4486 1104.8827 1103.6009 1.2818 0 14 37 1 ITAIITQGCK
260 342.6649 1024.9727 1024.6141 0.3586 1 14 32 1 RAVSLPLNR
127 412.5136 823.0127 823.3018 -0.2891 0 14 34 1 QDMNTEA + Oxidation (M)
93 346.6886 691.3627 690.3184 1.0443 1 14 44 1 SKSSADP
436 553.5482 1657.6227 1658.7648 -1.1421 1 14 27 1 RGYTASSLDSSSSWR
206 327.7715 980.2927 979.5464 0.7463 1 14 35 1 RHGLVGWR
31 327.4836 652.9527 651.3955 1.5572 1 14 27 1 TKIYK
405 757.9536 1513.8927 1515.7021 -1.8094 1 14 29 1 MATTATMATSGSARK + 2 Oxidation (M)
138 842.2000 841.1927 841.4657 -0.2730 0 14 35 1 SLALPADR
163 460.2436 918.4727 920.4273 -1.9546 0 14 43 1 CEIISDGK
239 340.7215 1019.1427 1019.4937 -0.3510 0 14 41 1 QHWNPPGGK
449 612.7315 1835.1727 1835.9894 -0.8167 1 14 26 1 RLTPPFNPNVTGPADLK
208 329.4082 985.2027 984.4512 0.7515 0 14 38 1 DHAAEVESK
318 387.6449 1159.9127 1160.5534 -0.6407 1 14 38 1 SEAGARGGGQGSK
391 484.6082 1450.8027 1449.7286 1.0741 1 14 29 1 FLMKLTSDGSHSK
210 494.3986 986.7827 988.4760 -1.6933 1 14 42 1 RDAGGPSMAK
371 448.9082 1343.7027 1343.7462 -0.0435 1 14 34 1 RIWVTAWVGTR
315 386.5615 1156.6627 1157.5764 -0.9137 1 14 38 1 WVGARMGEPR
401 502.6849 1505.0327 1503.7538 1.2789 1 14 30 1 RTVPPMVNVTCSK + Oxidation (M)
85 342.8086 683.6027 685.3871 -1.7844 1 14 39 1 GGARTPK
393 488.7849 1463.3327 1461.7796 1.5532 1 14 30 1 MVMIRGNSGLKPK + 2 Oxidation (M)
1 609.7600 608.7527 610.3187 -1.5660 0 14 31 1 QAGAHK
115 388.5936 775.1727 773.3668 1.8059 0 14 52 1 KPSGDDR
150 436.3136 870.6127 869.5446 1.0681 1 14 44 1 AELRLLR
194 488.7836 975.5527 974.4893 1.0634 1 14 44 1 SRQASVGDR
94 346.9386 691.8627 690.3813 1.4814 1 14 46 1 RYAPGK
282 522.0786 1042.1427 1042.6022 -0.4595 1 14 40 1 KVVVATNIAE
304 544.8086 1087.6027 1087.5444 0.0583 0 14 45 1 LTPNTCVQR
381 464.6815 1391.0227 1392.5479 -1.5252 0 14 33 1 VYYCGMDVWGQ + Oxidation (M)
288 529.8836 1057.7527 1056.5426 1.2101 1 14 46 1 EAFFKMLR + Oxidation (M)
380 460.4382 1378.2927 1379.7673 -1.4746 1 14 35 1 LPDPQALFPARR
233 340.6382 1018.8927 1017.5607 1.3320 1 14 45 1 PFSVVDARK
324 388.6115 1162.8127 1164.5961 -1.7834 0 14 39 1 IIAMSFPSSGR
433 552.8482 1655.5227 1654.9141 0.6086 0 14 29 1 LISEILSESVVPDVR
218 333.0782 996.2127 994.5084 1.7044 1 14 38 1 FVSGGSADKK
111 387.0236 772.0327 773.3854 -1.3527 1 14 54 1 GRQMAPV + Oxidation (M)
164 460.2436 918.4727 916.4879 1.9848 1 14 49 1 NVTYKHR
341 610.9036 1219.7927 1221.5594 -1.7667 0 14 41 1 KPGASMNVSCR + Oxidation (M)
384 464.8115 1391.4127 1391.6690 -0.2563 0 14 35 1 VISFHNCMIQK + Oxidation (M)
362 435.5915 1303.7527 1303.6349 0.1178 0 13 39 1 HPFALYGWGEK
417 520.7149 1559.1227 1560.8147 -1.6920 1 13 34 1 GEIFGLKAELNNEK
4 609.9300 608.9227 610.3187 -1.3960 0 13 35 1 QAGAHK
285 523.9236 1045.8327 1045.5954 0.2373 0 13 46 1 VAIMVSGTLR
186 484.6086 967.2027 966.4328 0.7699 0 13 40 1 MESTGELGK + Oxidation (M)
456 745.3682 2233.0827 2231.1281 1.9546 1 13 25 1 NLTSSSLNDISDKPEKDQLK
109 386.5586 771.1027 771.4603 -0.3575 1 13 51 1 AVRDALK
179 314.2349 939.6827 940.5355 -0.8528 1 13 40 1 GKPRAWAR
446 610.7615 1829.2627 1828.9539 0.3088 1 13 30 1 LIALCHSSGIKQDIMK + Oxidation (M)
120 398.4986 794.9827 796.3207 -1.3380 0 13 41 1 MASEGMR + Oxidation (M)
65 340.8486 679.6827 681.2639 -1.5812 0 13 42 1 DSGMEK + Oxidation (M)
98 358.4686 714.9227 713.4184 1.5043 0 13 63 1 AGGLALGR
105 365.4486 728.8827 729.4538 -0.5710 0 13 63 1 LFVPVR
73 683.5400 682.5327 682.3398 0.1929 0 13 41 1 SAPSGHK
314 385.9749 1154.9027 1156.6353 -1.7326 1 13 42 1 TEHKQFLVR
84 342.8036 683.5927 685.4123 -1.8195 1 13 47 1 PKTVNK
152 442.6836 883.3527 883.4109 -0.0582 1 13 43 1 KMISEGGY
290 533.7486 1065.4827 1066.5593 -1.0766 1 13 51 1 FQGRLSMTK
286 524.5436 1047.0727 1047.5713 -0.4986 1 13 50 1 RTVAAPSVFT
46 333.0786 664.1427 665.3166 -1.1739 0 13 57 1 MASSVR + Oxidation (M)
329 399.5149 1195.5227 1194.5564 0.9663 1 13 41 1 SSRTAWATCR
23 325.0136 648.0127 646.3286 1.6841 0 13 70 1 TGPSGTK
224 504.0836 1006.1527 1007.5036 -1.3509 1 13 48 1 AGGGGDAKTFK
30 327.4336 652.8527 654.3813 -1.5286 0 13 36 1 LGAAAPR
376 460.0015 1376.9827 1376.7161 0.2666 1 13 43 1 VNRGTQGVFGGGTK
325 388.6282 1162.8627 1163.6160 -0.7532 0 13 47 1 GAGLGAGGAHAVAR
374 685.9386 1369.8627 1371.6742 -1.8115 1 13 40 1 AKEAEGGAATAGPSR
385 464.8182 1391.4327 1392.6853 -1.2526 1 13 42 1 MQEMSRPINKK + 2 Oxidation (M)
422 523.8449 1568.5127 1569.7344 -1.2217 1 13 39 1 CSYIAESDAAVKEK
161 459.9986 917.9827 918.4117 -0.4290 0 13 58 1 MDVLSPGQG + Oxidation (M)
153 442.7036 883.3927 881.4355 1.9572 1 13 47 1 TYSGGNKR
346 414.8182 1241.4327 1240.5870 0.8457 0 13 47 1 VHCADLSNPTK
255 512.6286 1023.2427 1024.6103 -1.3676 0 13 47 1 AIMLGAKPPK
157 448.9086 895.8027 897.4192 -1.6165 0 13 47 1 SASSNFASK
155 448.8836 895.7527 897.4967 -1.7439 1 13 48 1 MHLIGRR + Oxidation (M)
117 388.6286 775.2427 776.3487 -1.1060 0 13 72 1 IMGDADR
231 510.3936 1018.7727 1020.6080 -1.8353 0 13 59 1 GVRPPAPSLK
420 522.0782 1563.2127 1562.8820 0.3307 0 13 41 1 AAPELAPAPFTLPLR
41 330.4986 658.9827 658.3034 0.6793 0 12 83 1 ADPNSR
45 331.8886 661.7627 661.3581 0.4046 1 12 79 1 KNAMAK
221 502.6836 1003.3527 1003.5008 -0.1481 0 12 60 1 AEEVVCVAK
37 327.7336 653.4527 654.3085 -0.8558 0 12 40 1 DLDHR
188 324.8549 971.5427 972.5465 -1.0038 1 12 60 1 RGTLAGGSVR
340 610.8236 1219.6327 1218.7309 0.9018 1 12 55 1 SRAVPIPARPR
257 512.9386 1023.8627 1022.5369 1.3258 1 12 51 1 HREAAGNLR
388 701.6436 1401.2727 1399.6164 1.6564 1 12 48 1 HGAHCGEHRTAPA
359 433.9515 1298.8327 1300.5830 -1.7503 1 12 49 1 CRTASHPVSASE
372 449.0249 1344.0527 1342.6009 1.4518 1 12 51 1 CKPMSYEEKR + Oxidation (M)
293 358.3282 1071.9627 1072.5335 -0.5708 0 12 63 1 MADGAPLAGVR + Oxidation (M)
437 558.8882 1673.6427 1673.7575 -0.1148 1 12 44 1 KSMIPSFMTMDQSR + Oxidation (M)
442 609.9415 1826.8027 1826.9171 -0.1144 1 12 37 1 IMKVPSMPPSQWPASR + Oxidation (M)
113 387.8236 773.6327 772.4443 1.1884 1 12 82 1 KAGLAADK
312 563.8886 1125.7627 1127.7026 -1.9399 1 12 55 1 KLSLSALGAIR
397 495.7515 1484.2327 1483.6799 0.5528 1 12 50 1 KIPDMYSEIGCR + Oxidation (M)
2 609.7700 608.7627 610.3187 -1.5560 0 12 48 1 QAGAHK
123 403.3336 804.6527 804.3687 0.2840 0 12 74 1 EDGMLPK + Oxidation (M)
121 399.5136 797.0127 796.4443 0.5684 0 12 53 1 QVPGTAPK
49 340.5186 679.0227 680.3493 -1.3266 0 12 62 1 EPPPNK
413 512.8115 1535.4127 1536.6562 -1.2435 1 12 48 1 CHECGKAFTQGSR
15 312.9636 623.9127 625.3296 -1.4169 0 12 65 1 ASHGVR
169 464.8136 927.6127 929.5043 -1.8915 1 12 64 1 LGERQATR
276 346.9382 1037.7927 1038.5206 -0.7279 1 12 56 1 QDTAKSPHR
421 522.6215 1564.8427 1565.8963 -1.0536 0 12 46 1 TTMDLHVLVIVGIR
170 464.8186 927.6227 926.4644 1.1584 1 12 64 1 MVYSSGKR
100 358.5036 714.9927 716.3891 -1.3963 0 12 87 1 MAAAPLK + Oxidation (M)
114 388.3586 774.7027 774.4487 0.2540 0 12 85 1 GTLVIVSS
57 340.6736 679.3327 681.3017 -1.9689 0 12 63 1 MHHNK + Oxidation (M)
297 358.5049 1072.4927 1070.5621 1.9306 1 12 67 1 RGFPLGGPDR
412 512.6315 1534.8727 1535.7250 -0.8523 0 12 50 1 MTQSPSTLAASVGDR + Oxidation (M)
283 522.6236 1043.2327 1042.5043 0.7284 0 12 65 1 GSSGPTSPTPR
309 553.5486 1105.0827 1103.5836 1.4991 0 12 66 1 ILHSHGSPTR
3 609.8300 608.8227 610.3187 -1.4960 0 12 52 1 QAGAHK
234 340.6415 1018.9027 1019.5148 -0.6121 1 12 72 1 GRAVDFAER
60 340.7186 679.4227 678.3561 1.0666 1 12 60 1 NRSFR
146 434.0536 866.0927 867.4702 -1.3774 0 12 61 1 GDILPPEK
12 311.6086 621.2027 619.3363 1.8664 1 12 94 1 MPTKK + Oxidation (M)
51 340.5636 679.1127 680.4836 -1.3709 0 12 69 1 ILPLLL
156 448.8886 895.7627 896.4062 -0.6435 0 12 60 1 CEAAFATK
249 341.2349 1020.6827 1020.3971 0.2856 0 12 67 1 CHDFSATSP
21 324.8536 647.6927 646.3762 1.3165 1 11 1e+002 1 RVSASK
424 524.5415 1570.6027 1568.8232 1.7795 1 11 54 1 TMLKDLATQTYIR + Oxidation (M)
181 476.6736 951.3327 951.3934 -0.0607 1 11 67 1 DDVERFDG
419 520.9715 1559.8927 1560.7242 -0.8315 1 11 54 1 VQKEDNSYYIMR + Oxidation (M)
300 360.6549 1078.9427 1077.5455 1.3973 1 11 67 1 GDQLFDQKK
358 433.7782 1298.3127 1298.7194 -0.4067 1 11 60 1 NKSGIISEPLNK
87 342.8336 683.6527 684.3304 -0.6776 0 11 72 1 GGPGGGGAR
335 609.9436 1217.8727 1217.6292 0.2435 0 11 69 1 VAVIQFSDDPK
131 415.0486 828.0827 827.3339 0.7488 0 11 85 1 MMSMNSK
167 464.7036 927.3927 928.4727 -1.0799 0 11 68 1 AAGVSPGTGGR
44 331.3586 660.7027 660.3555 0.3472 0 11 1.1e+002 1 SGLAGTR
306 552.8486 1103.6827 1104.5200 -0.8373 0 11 78 1 APSNEAGGAFGK
7 611.2000 610.1927 609.2540 0.9387 0 11 53 1 MSNSR + Oxidation (M)
416 520.7049 1559.0927 1559.6834 -0.5907 0 11 59 1 VCHHADCQQLHR
427 530.1082 1587.3027 1585.7810 1.5217 0 11 61 1 IGLFYMDNDLITR + Oxidation (M)
5 610.0100 609.0027 610.3187 -1.3160 0 11 61 1 QAGAHK
58 340.6936 679.3727 680.3493 -0.9766 0 11 74 1 EPPPNK
457 757.9515 2270.8327 2270.1769 0.6558 1 11 39 1 SQHELLFSLTQICIKYYK
458 1736.8486 3471.6827 3469.7681 1.9146 0 11 19 1 HVLGTVGMCILIMQILGMAFSMTLFQHIHR + Oxidation (M)
291 533.8236 1065.6327 1064.5403 1.0924 0 11 82 1 IYHTNYVR
103 360.6536 719.2927 717.3769 1.9158 1 11 1e+002 1 RDASAAK
36 327.6636 653.3127 651.3340 1.9787 0 11 55 1 EPGPPR
320 581.5036 1160.9927 1162.5288 -1.5361 1 11 81 1 DEIEKQMDR
402 503.0915 1506.2527 1506.7534 -0.5007 0 11 66 1 TPSASGLMVLASAMR + Oxidation (M)
187 485.4286 968.8427 968.4967 0.3460 1 11 72 1 EKEAAFFK
209 493.9786 985.9427 986.5621 -0.6194 1 11 87 1 ASSVLAQRR
447 610.8249 1829.4527 1828.9584 0.4943 0 11 54 1 LYGQTNFSPIINHVAR
82 342.7436 683.4727 685.4123 -1.9395 1 11 84 1 PKTVNK
418 520.7882 1559.3427 1560.8511 -1.5084 1 11 66 1 LPEPSASLPNPPSKK
62 340.7736 679.5327 681.4425 -1.9098 0 10 82 1 LNPLLI
28 327.0786 652.1427 651.3010 0.8417 0 10 77 1 MTTAGR + Oxidation (M)
24 325.0736 648.1327 646.2744 1.8583 0 10 1.4e+002 1 DEPMR
91 343.1286 684.2427 685.3507 -1.1080 0 10 94 1 SAAPGQR
274 343.1282 1026.3627 1027.5047 -1.1419 0 10 86 1 LGHGNSSTQK
278 520.7036 1039.3927 1037.5254 1.8673 0 10 86 1 QSSHLTTHK
299 360.6415 1078.9027 1077.5237 1.3791 1 10 92 1 MAEERVATR + Oxidation (M)
250 511.6936 1021.3727 1022.4781 -1.1054 1 10 96 1 GQYQDKER
68 340.9586 679.9027 681.3553 -1.4526 1 10 91 1 LMKMK + 2 Oxidation (M)
215 331.0315 990.0727 989.4059 0.6668 0 10 1.1e+002 1 GHTSPCGMK + Oxidation (M)
148 435.5936 869.1727 870.4671 -1.2944 1 10 91 1 SLKSHSGR
363 435.7182 1304.1327 1303.6448 0.4879 1 10 92 1 AFDDIAKYFSK
38 327.7736 653.5327 653.3497 0.1830 0 10 73 1 GTGLLGH
54 340.6386 679.2627 677.3344 1.9283 0 10 1e+002 1 GSSSGGVK
423 523.9249 1568.7527 1566.7712 1.9816 1 10 79 1 YEQMKQQEVQLK + Oxidation (M)
70 341.2336 680.4527 680.2435 0.2092 0 10 88 1 GGNESAM + Oxidation (M)
168 464.7236 927.4327 927.5290 -0.0963 1 10 98 1 HKILFDR
128 412.6536 823.2927 823.4626 -0.1698 1 10 99 1 MAFLAKK + Oxidation (M)
13 311.7836 621.5527 621.3235 0.2293 1 10 1.3e+002 1 FVGRSG
64 340.8436 679.6727 679.3905 0.2823 0 9 1e+002 1 SFSLVK
375 459.1449 1374.4127 1374.7844 -0.3717 1 9 95 1 GALAQGKAPRPGPR
92 689.6700 688.6627 687.3738 1.2889 0 9 1.6e+002 1 MVPAVR + Oxidation (M)
441 609.9082 1826.7027 1824.9669 1.7359 1 9 72 1 MFLPSLGHRAPVSQAAK + Oxidation (M)
42 330.9286 659.8427 658.3875 1.4553 1 9 1.8e+002 1 RVGTAR
67 340.9336 679.8527 679.2748 0.5779 0 9 1e+002 1 WGDMR + Oxidation (M)
326 388.7982 1163.3727 1164.5081 -1.1353 0 9 1.1e+002 1 GLEGSEMESAR
132 418.6686 835.3227 836.4214 -1.0987 1 9 1.6e+002 1 KSWAMSK
35 327.6286 653.2427 654.3085 -1.0658 0 9 91 1 DLDHR
99 358.4686 714.9227 716.3705 -1.4477 0 9 1.7e+002 1 VSPESAK
277 520.5536 1039.0927 1039.5298 -0.4371 0 9 1.2e+002 1 AAPEAPTLDR
410 512.1182 1533.3327 1532.8198 0.5129 0 9 1.1e+002 1 EFALRPEGSVSTLK
17 314.7836 627.5527 629.3748 -1.8221 0 9 1.5e+002 1 ALLEGK
32 327.5136 653.0127 652.3544 0.6583 1 9 99 1 SGKAYK
124 403.6136 805.2127 806.3882 -1.1755 1 8 1.7e+002 1 GSESKSGR
256 512.8136 1023.6127 1023.4920 0.1207 1 8 1.3e+002 1 GVCGFRGGSK
43 331.0336 660.0527 661.3143 -1.2616 0 8 2.4e+002 1 GGQSSAR
11 621.6800 620.6727 621.2792 -0.6065 0 8 2.2e+002 1 DGATMK
281 520.9736 1039.9327 1039.5345 0.3982 1 8 1.4e+002 1 LICGREHR
22 324.9886 647.9627 646.3472 1.6155 0 8 2.3e+002 1 MAGKPK + Oxidation (M)
59 340.7086 679.4027 678.3853 1.0174 0 8 1.5e+002 1 IPFFR
56 340.6536 679.2927 677.3344 1.9583 0 8 1.6e+002 1 GSSSGGVK
303 544.0736 1086.1327 1086.5604 -0.4277 1 8 1.8e+002 1 MSPSRLPGSR
80 342.7136 683.4127 683.3239 0.0889 0 7 1.6e+002 1 STDHPK
102 360.6436 719.2727 718.4225 0.8502 0 7 2.2e+002 1 VLATSTK
133 418.7236 835.4327 836.4980 -1.0653 1 7 2.3e+002 1 VPPAAARR
34 327.6036 653.1927 651.2534 1.9394 0 7 1.3e+002 1 DEMNK + Oxidation (M)
50 340.5586 679.1027 679.2959 -0.1932 0 7 1.9e+002 1 MGSDVR + Oxidation (M)
139 844.3800 843.3727 841.4446 1.9281 0 7 2.2e+002 1 AAFPPSPR
280 520.7886 1039.5627 1041.5389 -1.9762 1 7 1.6e+002 1 GNKPHKSMK + Oxidation (M)
173 932.3900 931.3827 930.5134 0.8693 1 7 2.1e+002 1 VPSKSNTAK
302 542.5686 1083.1227 1084.5989 -1.4761 1 7 1.8e+002 1 DIRAALAAER
69 340.9886 679.9627 681.3190 -1.3562 1 7 1.9e+002 1 MKLMSG + Oxidation (M)
118 388.7986 775.5827 775.4262 0.1565 0 7 2.6e+002 1 IANAIMK + Oxidation (M)
450 1858.5200 1857.5127 1856.9607 0.5520 0 7 1.4e+002 1 MDDWKPSPLIKPFGAR
8 611.2200 610.2127 611.3391 -1.1264 1 6 1.6e+002 1 EKAHK
101 360.5536 719.0927 719.3086 -0.2159 0 6 2.9e+002 1 NNTEDK
72 342.0586 682.1027 680.2878 1.8149 0 6 1.8e+002 1 NNDYR
25 325.2236 648.4327 649.2854 -0.8526 0 6 3.1e+002 1 NGATCK
52 340.5736 679.1327 680.2799 -1.1472 0 6 2.7e+002 1 MSDANK + Oxidation (M)
9 615.1400 614.1327 612.2611 1.8716 1 5 3.4e+002 1 ATKMM + 2 Oxidation (M)
89 342.9586 683.9027 685.3620 -1.4592 1 5 3.1e+002 1 GPRGGSR
6 610.1800 609.1727 610.3187 -1.1460 0 4 2.8e+002 1 QAGAHK
451 1893.7200 1892.7127 1890.8959 1.8168 0 3 3.5e+002 1 EQDVDVSSHSYTINGLK
16 314.2336 626.4527 627.2976 -0.8449 0 2 4.5e+002 1 SHAGEK
452 1918.8000 1917.7927 1917.9692 -0.1765 1 2 4.3e+002 1 DTCTRMYIVALFTIAK + Oxidation (M)
453 1990.4600 1989.4527 1989.0102 0.4425 1 1 4.1e+002 1 NNNARLMFGDGTQLVVKP + Oxidation (M)
47 669.0500 668.0427 669.3810 -1.3382 0 1 6.1e+002 1 VQQPAK
140 846.0000 844.9927 843.4749 1.5178 1 0 1.2e+003 1 RVLMGPR + Oxidation (M)
--------------------------------------------------------------------------------
Search Parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Fixed modifications : Carbamidomethyl ©
Variable modifications : Oxidation (M)
Mass values : Monoisotopic
Protein Mass : Unrestricted
Peptide Mass Tolerance : ± 2 Da
Fragment Mass Tolerance: ± 0.8 Da
Max Missed Cleavages : 1
Instrument type : ESI-TRAP
Number of queries : 458
thanks
Hi
U should read the mascot tutorial on the web for a complete explanation but as a beginer, this would help
Just focus on "Individual ions scores > 42 indicate identity or extensive homology (p<0.05)."
So above 42 all hits are good and can be considered. One should be more confident when the score is much greater than this number. So something in 60s or even in 100s is not a very reliable result but can be taken with caution.
I remember sequencing one of my protein where the cut off value was 25 (42 for you) and the score was 7001.
But a score of say 500 would have been good enough to take the result with confidence. But this was a recombinant protein and the conc was very high and i used a licensed version of mascot. For other proteins i like to confirm teh mass spec sequencing results with N-terminal sequencing or proteolytic cleavage after mass spec based identification.
Results in order of decreasing scores are mentioned with the peptides that have been identified. If u click on any of the results, it will tell u the percentage coverage of teh protein.
Hope it helps or u can get back with specific questions.
TC
Thanx for your kind suggestion
Is the mascot tutorial avialable on the google.............. if you have that tutorial then please mail to me...............
thanx so much.............
Hi
Its freely available online. You can surf here:
https://www.matrixscience.com/help/interpretation_help.html
TC