Troubleshooting! Strange growth in LB medium - (Nov/12/2008 )

I've been having a bit of trouble with cloning the past few weeks. I get good colony growth on my lb plates, but after I pick the colonies and incubate them in lb media overnight, I get (what I think is) genomic dna growth on the toothpicks, but the medium is not cloudy... I've tried to go on with restriction enzyme digest with this solution + dna, but all I end up with is a blank gel.

Please let me know if you have any solutions to this! Thank you!

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Refer to this topic, maybe it can help: http://www.protocol-online.org/forums/inde...amp;hl=wolfgang
Are you using E. coli? If so, it's ALWAYS CLOUDY.

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If your is not a language problem, i think you need to work on your basics. It doesn't make sense at all, to me.

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Are you saying that you get a lot of stuff that sticks to your toothpick after overnight culture? Could be that your cells are sticking to the toothpick and growing there without really getting into the media. I usually use sterile pipet tips myself, but you could try to vortex your cultures slightly before incubating them to get them into the solution better.

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