Why use 200 units of ligase? - I am going cuckoo lol (Oct/10/2008 )

Add the equilibration buffer and shake the bottle. Allow the phases to separate. Leave the aqueous layer. Do not bother remixing the two layers after that. Pipet phenol or phenol:chloroform from below the aqueous layer. Store at 4C to slow down oxidation.

yup!! thanks!!