Cutting out EGFP gene from pEGFP-N1 - w/o using XbaI (Jul/19/2008 )
anyone who ever cut out EGFP gene without using XbaI at 3´ of pEGFP-N1 vector?
-The Bearer-
i pcr'd it up with primers tagged with my restriction enzymes of interest.....cheating i guess, but easier!
QUOTE (The Bearer @ Jul 20 2008, 07:23 AM)
anyone who ever cut out EGFP gene without using XbaI at 3´ of pEGFP-N1 vector?
-aussieuk-
I used NotI...
-stardust-