what is more important in protein electrophoresis? voltage or current? - sds-page (Apr/11/2008 )
QUOTE (Felix_the_labcat @ Apr 25 2008, 12:38 PM)
I ran an empty gel and the current behaviour was the same 
So something in the gel migrates too, creating the current.
There are two questions
a)what is creating the current
b)do we want/need it to migrate along with our samples?
(in case of sds molecules)
So something in the gel migrates too, creating the current.
There are two questions
a)what is creating the current
b)do we want/need it to migrate along with our samples?
(in case of sds molecules)
the current is electric current. what you are seeing migrate, in a blank gel, is a front (glycine or tricine) from the reservoir buffer.
-mdfenko-