DC Protein Assay - (Feb/01/2008 )
HI!
I am currently using DC Protein Assay from Bio-Rad which comes together with Reagent A, Reagent B and Reagent S.
I would like to ask whether i can prepare my own Reagent S since the common name is sodium dodecly sulfate as mentioned in the manual.
Any idea how to prepare it?
Thank you. 
-mimosa-
QUOTE (mimosa @ Feb 1 2008, 03:20 PM)
HI!
I am currently using DC Protein Assay from Bio-Rad which comes together with Reagent A, Reagent B and Reagent S.
I would like to ask whether i can prepare my own Reagent S since the common name is sodium dodecly sulfate as mentioned in the manual.
Any idea how to prepare it?
Thank you.
I am currently using DC Protein Assay from Bio-Rad which comes together with Reagent A, Reagent B and Reagent S.
I would like to ask whether i can prepare my own Reagent S since the common name is sodium dodecly sulfate as mentioned in the manual.
Any idea how to prepare it?
Thank you.
Hello
I think it is OK that you prepare your own reagent S, considering that the reagent is sodium dodecyl sulfate (SDS) at 5-10% in water. Considering a 5% solution and that 5 mL are considered sufficient for 500 standard assays or 10,000 microplate assays, then you need to dissolve 0.25 g SDS in 5 mL of water...
-Jellyfish-
QUOTE (Jellyfish @ Feb 4 2008, 05:58 AM)
QUOTE (mimosa @ Feb 1 2008, 03:20 PM)
HI!
I am currently using DC Protein Assay from Bio-Rad which comes together with Reagent A, Reagent B and Reagent S.
I would like to ask whether i can prepare my own Reagent S since the common name is sodium dodecly sulfate as mentioned in the manual.
Any idea how to prepare it?
Thank you.
I am currently using DC Protein Assay from Bio-Rad which comes together with Reagent A, Reagent B and Reagent S.
I would like to ask whether i can prepare my own Reagent S since the common name is sodium dodecly sulfate as mentioned in the manual.
Any idea how to prepare it?
Thank you.
Hello
I think it is OK that you prepare your own reagent S, considering that the reagent is sodium dodecyl sulfate (SDS) at 5-10% in water. Considering a 5% solution and that 5 mL are considered sufficient for 500 standard assays or 10,000 microplate assays, then you need to dissolve 0.25 g SDS in 5 mL of water...
Hi Jellyfish!
Thank you very much for ur advise.
Hopefully it does work as well as the commercially bought Ragent S!
-mimosa-
You are welcome! I hope it works.
Bye! 
-Jellyfish-