Centrifugation speed for adherent cell line - (Jan/14/2008 )
I would suggest a longer time but with less rpm/g
I've never centrifuged less than 10 minutes and never more than 1200 rpm. Don't know precisely the radius of the centrifuges I used, but they were pretty big.
Hope it helps!
-panda-
QUOTE (panda @ Jan 14 2008, 11:10 AM)
I would suggest a longer time but with less rpm/g
I've never centrifuged less than 10 minutes and never more than 1200 rpm. Don't know precisely the radius of the centrifuges I used, but they were pretty big.
Hope it helps!
I've never centrifuged less than 10 minutes and never more than 1200 rpm. Don't know precisely the radius of the centrifuges I used, but they were pretty big.
Hope it helps!
Ya...I agree with you. I think 125g -275g for 5 min is the range of centrifugation force for adherent cells.
-sasoriza-