Loss of LMW in Protein Standard - (Aug/29/2007 )

Throughout the past 6 months I have been losing LMW (15 and 10kDa) protein standard in our chemilumiscent ladder from Bio-Rad. They used to appear after atleast 5s of developing time. Now we are lucking if we see them at 5min or 10min. We know it is not a probelm with streptactin. We are currently inspecting our transfer apparatus becuase we found out the leads were corroded to some degree with the transfer buffer salts. My question is --Has anyone else encountered this problem? Is there anything else that may be responsible for this phenomenon? Thank you for any suggestions.

-wunfuntin-

You can load some regular LMW markers, do the transfer and stain with a dye called Ponceau S. This will be the easiest way to check if you have transfer problem.

Did you store your marker at -20 C?

-genehunter-1-

QUOTE (genehunter-1 @ Aug 29 2007, 02:34 PM)

You can load some regular LMW markers, do the transfer and stain with a dye called Ponceau S. This will be the easiest way to check if you have transfer problem.

Did you store your marker at -20 C?

We do store our marker at -20 C. Thank you for your suggestion. That may be my next step if my test experiment this week does not work.

-wunfuntin-