Different size protein then expected - (Aug/10/2007 )
Hi
I am trying to charaterize a viral protein which is encoded by two Exons in viral genome.
Size of that protein should be about 75 Kda according to protein sequence.
But this protein show 50 Kda band in western and radiolabelling of viral infected cells, when probed with Abs, which are targeted to C terminal of protein.
Further I cloned cDNA of same in mammalian expression vector but I still got the same size band.
Can any one speculate what could be happening there?
-ahuja-
QUOTE (ahuja @ Aug 11 2007, 02:55 AM)
Hi
I am trying to charaterize a viral protein which is encoded by two Exons in viral genome.
Size of that protein should be about 75 Kda according to protein sequence.
But this protein show 50 Kda band in western and radiolabelling of viral infected cells, when probed with Abs, which are targeted to C terminal of protein.
Further I cloned cDNA of same in mammalian expression vector but I still got the same size band.
Can any one speculate what could be happening there?
I am trying to charaterize a viral protein which is encoded by two Exons in viral genome.
Size of that protein should be about 75 Kda according to protein sequence.
But this protein show 50 Kda band in western and radiolabelling of viral infected cells, when probed with Abs, which are targeted to C terminal of protein.
Further I cloned cDNA of same in mammalian expression vector but I still got the same size band.
Can any one speculate what could be happening there?
how is the size of polypeptides if each exons will be separately expressed? Would one of the exons coding for a polypeptide of ~50 kDa?
-The Bearer-
Any possibility of it being cleaved ? Or a second start site resulting in a shorter version?
-scolix-