peptide phosphorylation analysis method - (May/09/2007 )
Hi,everyone.
I would like to ask about the peptide phosphorylation analysis methods.
Now, I have to search substrate peptides for certain kinases,and mainly use mass spectrometry for phosphorylation analysis.
But sometimes i got different results for the same substrate...
Please tell me some other methods for peptide phosphorylation analysis.
-hana_angel-
QUOTE (hana_angel @ May 10 2007, 06:45 AM)
Hi,everyone.
I would like to ask about the peptide phosphorylation analysis methods.
Now, I have to search substrate peptides for certain kinases,and mainly use mass spectrometry for phosphorylation analysis.
But sometimes i got different results for the same substrate...
Please tell me some other methods for peptide phosphorylation analysis.
I would like to ask about the peptide phosphorylation analysis methods.
Now, I have to search substrate peptides for certain kinases,and mainly use mass spectrometry for phosphorylation analysis.
But sometimes i got different results for the same substrate...
Please tell me some other methods for peptide phosphorylation analysis.
to be precise: you are interested in phosphopeptide analysis of phosphorylated proteins
or (in vitro methods) of phosphorylation of peptides or both?
-The Bearer-
I just want to analyze the phosphorylation of peptides.
-hana_angel-
QUOTE (hana_angel @ May 10 2007, 12:10 PM)
I just want to analyze the phosphorylation of peptides.
most simple is utilizing peptide libaries f.i. spotted, which are incubated with your kinase (or kinase phosphatase system) of interest
-The Bearer-