How do I choose a 96-well plate for BCA assays? - (May/08/2007 )

Hi there,

I'm a DNA person who is slowly making the transition to studying protein expression. I'm planning on doing BCA assays to quantify the protein I've isolated in 96-well format, as I have a LOT of samples to analyse. However, I never imagined that purchasing 96-well plates for my assays would prove to be the most confusing part of my experiment so far! I'm totally overwhelmed at the variety of 96-well plates available, and I have no idea which ones to buy.

Obviously, I need to choose plates with an optical bottom, but beyond that, does anyone have any advice on what to look for?

For example:

-clear, black or white plates?
-type of plastic?
-protein-binding capacity of the wells? (such as maxisorp, midisorp, etc. from Nalgene)

Thanks in advance,

Ginger

I use clear polistyrene, without any fancy modifications for protein binding (those are rather for ELISA).

In our case we using flat bottom polystyrine 96 well culture plate for BCA reading. I think its will be fine . there is no need to purchase black or conical bottom 96 well culture plate.

Thanks for the responses. I didn't think I needed anything special, but it's good to have some confirmation.

Ginger

hi,

polyvinyl chloride plates are also ok

Sebastien