E coli protein extraction for EMSA - (Mar/30/2007 )
Hi everybody,
I want to extract protein from e coli cells in order to do EMSA. If I sonicate in a HEPES 25mM, NaCl 200mM, NP-40 0,01%, protease inhibitor buffer, is it fine??
Thanks.
-Fredo-
Should be OK, give it a shot.
-swanny-