Voltage and running time for IEF - (Mar/27/2007 )
i have read some posts here stated that a larger voltage with longer running time and larger pH gradient would lead to a great resolution for the IEF gel. can someone explain for me what is the point behind the claim of the voltage factor? because what i have heard form the lecture that...........
when the voltage is large, the ohmic heat generates and denatures some of the sample. this will alter the diffusion rate of some of them and cause poor resolution.
may any one kindly point out my misunderstanding? thanks a lot!
-meaningless-
QUOTE (meaningless @ Mar 28 2007, 01:14 AM)
i have read some posts here stated that a larger voltage with longer running time and larger pH gradient would lead to a great resolution for the IEF gel. can someone explain for me what is the point behind the claim of the voltage factor? because what i have heard form the lecture that...........
when the voltage is large, the ohmic heat generates and denatures some of the sample. this will alter the diffusion rate of some of them and cause poor resolution.
may any one kindly point out my misunderstanding? thanks a lot!
when the voltage is large, the ohmic heat generates and denatures some of the sample. this will alter the diffusion rate of some of them and cause poor resolution.
may any one kindly point out my misunderstanding? thanks a lot!
you may run a ramp up to 2000 V but will not start directly with 2000 V; exact conditions depend on your system; follow the instructions of the manufacturer of IEF strips
-The Bearer-