TA cloning help - (Dec/12/2006 )

Hi there,
Well I had very bad experience with clony PCR. Thats' why I switched to digestion.
(PCR didn't work for me while, digestion was perfect when I checked the same colonies)

One more thing, while doing TA cloning I noticed something strange. When I use Taq to amplify my inserts I got some extra bases either at the end or at the beginning. So, I tried expand long plymerase and it worked very well.

Thanks,

Taq does tail its products. That is probably where the extra base pairs are coming from.