Purpose of washing column using PBST and PBS - (Nov/20/2006 )

Hi,

I would like to understand and know what is the purpose of washing the Poly-prep chromatography column by PBST and PBS?

-aiz-

QUOTE (aiz @ Nov 21 2006, 08:24 AM)

Hi,

I would like to understand and know what is the purpose of washing the Poly-prep chromatography column by PBST and PBS?

to wash out unspecific bindings, equilibration, and blocking of unspecific binding sites with Tween

-The Bearer-