Diluting triton in water - (Sep/08/2006 )
you can go up to 10% at least.
-Missele-
I start with a rapidly-stirring volume of water in an overlarge container (so it won't jump the sides if you turn up the magnetic stirrer)
drizzle the triton in. leave it for a while. it will go into solution, shouldn't take too long if your lab is pretty warm
I think it's harder to measure the volume than it is to get it into solution
-aimikins-
QUOTE (aimikins @ Sep 11 2006, 11:17 AM)
I start with a rapidly-stirring volume of water in an overlarge container (so it won't jump the sides if you turn up the magnetic stirrer)
drizzle the triton in. leave it for a while. it will go into solution, shouldn't take too long if your lab is pretty warm
I think it's harder to measure the volume than it is to get it into solution
drizzle the triton in. leave it for a while. it will go into solution, shouldn't take too long if your lab is pretty warm
I think it's harder to measure the volume than it is to get it into solution

I agree about it being harder to measure.
I do the same thing when diluting triton.
-jamie419-
QUOTE (swanny @ Sep 11 2006, 02:17 PM)
Go with genehunter. I would typically make up 50 ml by adding the required MilliQ volume, nuking it for 30 sec, then adding the Triton and inverting a few times. Hey presto, Triton stock in about 1 minute!
Well, if you're going to start a competition...

I'd usually make 25% Triton by the "microwave and add Triton" method.
-swanny-
Yes, make a high concentration stock and dilute from it seems to be the most flexible and time-saving way to prepare working solution at any concentration that you want.
-genehunter-1-