Diluting triton in water - (Sep/08/2006 )

I wanted to make a 5% triton solution so I diluted 10 ml pure triton into 190 ml water but I get a solid transparent precipitate...
How do I get rid of this precipitate?

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u need to vortex it well. Triton takes a while to dissolve.

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If you don't want to have a lot of foam, you can also rotate it ON

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Thanks!
I was wondering if I had done anything wrong cause the triton did not seem to dislove much at first but now it is slowly dissolving while rotating.

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we always stir with a magnetic stir bar.

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I like to microwave briefly to help desolving it.

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What's wrong with the classic technique of shaking really hard?

If you plan ahead you can shake all you want and leave the solution overnight for the bubbles to disappear.

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it takes more effort. why shouldn't we use labor saving devices like stirrers, mixers, shakers, etc.? that's what we bought them for.

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Go with genehunter. I would typically make up 50 ml by adding the required MilliQ volume, nuking it for 30 sec, then adding the Triton and inverting a few times. Hey presto, Triton stock in about 1 minute!

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Old school.

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