biotinylated proteins purification from free biotin - dialysis vs gel filtration or what else (Jul/19/2006 )

For some routine biotinylations we have conducted here at work, we always separate the product from free biotin using a PD10 column. They always work well, and the fractions of interest usually elute in 1ml, from a loading volume of 0.5ml. Dilution of the sample is something you will have to live with if you choose this method, but its quick and reliable.