making large proteins - (Jul/13/2006 )

Does anyone have any preferred methods for large protein synthesis(~100kDa) that they would like to share or discuss? If not could anyone direct me to any info on this subject? Thanks.

可以尝试原核表达, 使用pQE30或pET28载体

hi
i use baculovirus system for 150 routinely and up to 250kD production

Thanks for your help, but I can't translate your suggestion into english. Something about pQE30 and pET28 antibodies?

Thanks!!