extracellular domain from a transmembrane protein, secreted or not? - (May/23/2006 )
I clone and express a whole length of extracellular part from a transmembrane protein, inculding its signal peptide. Will this truncated protein be secreted into medium?
-macrosky-
QUOTE (macrosky @ May 24 2006, 07:37 AM)
I clone and express a whole length of extracellular part from a transmembrane protein, inculding its signal peptide. Will this truncated protein be secreted into medium?
Have you tried concentrating the supernatant? You could just do a quick TCA pptn, and run a gel to see if your protein is there.
-swanny-
How to concentrate the supernatant? What is quick TCA pptn? I have little experience with protein purification thing. Could you give me some details or reference? Thank you very much.
QUOTE (swanny @ May 23 2006, 11:28 PM)
QUOTE (macrosky @ May 24 2006, 07:37 AM)
I clone and express a whole length of extracellular part from a transmembrane protein, inculding its signal peptide. Will this truncated protein be secreted into medium?
Have you tried concentrating the supernatant? You could just do a quick TCA pptn, and run a gel to see if your protein is there.
-macrosky-