absorption spectroscopy - (Mar/21/2006 )

given that the absorption of a lmg/ml protein solution at 280nm is 1.3, how can I find the extinction coefficient?

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Hey

What you want is beer's law:

A (absorption at wavelength) = e * c * l

e = extinction coefficient
c= concentration of sample
l = path length of light through your sample in cm.

This is merely the width of the cuvette you were using to measure your sample in. I usually use 1cm cuvettes so this makes the calculation nice and easy!

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