Removal of LPS - (Mar/17/2006 )

Hi

I have a protein sample that are contaminatet with LPS I plan on remoweing it on an ionexchange colum. Is that wise.

Best regards Prisme

Methods how you can remove endotoxins are:
 Heat sterilization
 Treatment of strong acid or alkali
 Adsorption on activated carbon, asbestos or barium sulphate
 Distillation
 Reverse osmosis
 Ultrafiltration
 Two-phase extraction
 Adsorption technique
- Ion-exchange chromatography
- Affinity chromatography (e.g. EndoTrap)
- Size-exclusion chromatography
- Reverse-phase chromatography