What media would promote the best E.coli growth at a low cost for fermentation? - (Nov/22/2005 )

The only thing I can suggest is using LB and making the innoculm for the fermentor up from say a litre of cells (say 20 flasks of 50 mL). This way the cells won't need to go through many doublings to get to the density you want.

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what are the components of LB?
and also, I cant do that.. I have to grow up the cells in a 200ml shakeflask, then transfer to 9.8L fermenter. 8 hours begin once i innoculate shakeflask

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LB is just tryptone, yeast extract and salt. Just grow multiple 200ml Flasks.

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cant do that either
one 200ml shakeflask for inoculum, 9.8L fermenter medium

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Well good luck then

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so you guys think i should use lb?

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so you guys think i should use lb?

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so you guys think i should use lb?

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Yes.

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