Bacteria Science Fair Questions - A few basic and complex questions about bacteria (Nov/04/2005 )
Yes.
I'm not sure if I've said this before, or had a cleared "OK" on this idea, but instead of putting drops of antibacterials/disinfectants, can I put a small, circular piece of paper towel/coffee filter, soaked with the antibacterial/disinfetant? Thanks
Yes, that will work well -- provided the disks are sterile and you handle them sterily. You can probably buy some disks (like these 1/2 inch disks or these 1/4 inch disks), but I don't know how much these are, or whether they come pre-sterilized.
Yes, that will work well -- provided the disks are sterile and you handle them sterily. You can probably buy some disks (like these 1/2 inch disks or these 1/4 inch disks), but I don't know how much these are, or whether they come pre-sterilized.
Great, but they are labled "antibiotic-assay discs", that doesn't mean they already have an antibiotic planted in them, does it?
I've just found these assortment of different diameter discs, at http://216.15.207.230/cat/prodprice2_Detail.cfm?ID=779 , which I think are sterile, and are about $22.00 for 1000. Will these be fine?
Hi
I think is very nice you want to do such a study but I believe you will spend a lot of time and still you might encounter some problems.
I assume in your University there is Microbiology course or class? if yes why you don´t talk to responsible teacher then. If he cannot help you for sure he knows to whom you should talk.
it would so much easier and less time consuming if you would work in colaboration with other people, where growing bacteria was already currently done, where there autoclaves and all material required.
If you safe time and money by ordering less stuff, you will more time to repeat experiement, test different chemicals, analyse your results and write a great report.
make sure you are following all lab safety rules!!!!!!!!!
Ask the help of someone experience in microbiology.
You need a tutor!
No, there are no antibiotics on these, they're just paper disks.
Those look to be about the same as the disks I found, and should be fine, provided they come pre-sterilized or you have a way to sterilize them.
I think is very nice you want to do such a study but I believe you will spend a lot of time and still you might encounter some problems.
I assume in your University there is Microbiology course or class? if yes why you don´t talk to responsible teacher then. If he cannot help you for sure he knows to whom you should talk.
it would so much easier and less time consuming if you would work in colaboration with other people, where growing bacteria was already currently done, where there autoclaves and all material required.
If you safe time and money by ordering less stuff, you will more time to repeat experiement, test different chemicals, analyse your results and write a great report.
make sure you are following all lab safety rules!!!!!!!!!
Ask the help of someone experience in microbiology.
You need a tutor!
I am asking the local hospital to use their facilities, so it will be a safer environment than my house, hopefully they will accept
I've just contacted eNasco about their MicroLIVE Bacteria Cultures and they said the tube was only meant for one culture ( http://www.enasco.com/Search.do?searchTerm=microLIVE ); so I guess I will have to populate the bacteria, let it grow for a while, then take samples from that like we said before, because I don't want to spend $150+ on just the E. coli colonies.
There is a terminology problem here -- each tube is a culture; a culture is any volume of pure bacteria growing in media. You only need one tube of each organism. The question was what the volume of the culture is in each individual tube was (0.5 ml? 1 ml?), so we could guess whether there was enough liquid in a single tube for 15 Q-tips full.
It's not a problem to first streak the cultures to a plate and use that plate as an inocculum source for all the others after it has grown for a day.