Ligation is killing me - 1kb insert, 6 kb vector (Nov/01/2005 )

Hi again,
Sorry only now I realised you already solved your problem.
I am a bit distracted.
Good you found out the problem.

still, for anyone else reading this .... add at least 6 bases, some enzymes need even more. in my lab we had this problem last month. if possible, a larger extention is quite useful, because you can check on a gel, if restriction enzymes did their job