2D electrophoresis - beta-lactoglobulin (Jul/16/2009 )
Hello,
I was reading a protocol to prepare cell lysates to be analyzed by 2D-electrophoresis. The protocol describes spiking of the lysate with beta-lactoglobulin A. Is this step necessary? and how does it help?
Best,
Tarun
-T C-
My mistake...they were spiking it with beta-lactoglobulin to detect and see if their method actually differentiates between control and spiked samples (it was an exp)
How do I delete a topic?
Best,
TC
T C on Jul 16 2009, 02:30 PM said:
Hello,
I was reading a protocol to prepare cell lysates to be analyzed by 2D-electrophoresis. The protocol describes spiking of the lysate with beta-lactoglobulin A. Is this step necessary? and how does it help?
Best,
Tarun
I was reading a protocol to prepare cell lysates to be analyzed by 2D-electrophoresis. The protocol describes spiking of the lysate with beta-lactoglobulin A. Is this step necessary? and how does it help?
Best,
Tarun
-T C-