Storage of 8M Urea buffer? - I made it two weeks ago? (Jun/24/2009 )
Hello,
I made a some 8M Urea buffer two weeks ago and since then Ive had it at room temperature. I read somewhere that u used always make it fresh and/or store it at 4C? Is this true then I cant use my big bottle of buffer 
I hope someone can tell me this isnt true hehe
Oh and my buffer consists of Urea, Tris and NaH2PO4 in case it makes a difference
What u heard is true and will affect denaturing purification (I am assuming that is what u plan to do with the buffer)- to what extent, can be judged by doing a mini-scale purification.
Not recommended to pre-make urea containing solutions- u can try salvaging what u have if it still works in ur application.
Urea in water releases isocyante, which will carbamylate yr proteins. Make fresh buffer. Read this:
http://www.ionsource.com/Card/carbam/mono0005.htm
Best,
TC
PhD on Jun 24 2009, 06:38 PM said:
I made a some 8M Urea buffer two weeks ago and since then Ive had it at room temperature. I read somewhere that u used always make it fresh and/or store it at 4C? Is this true then I cant use my big bottle of buffer
I hope someone can tell me this isnt true hehe