LPS isolation by hot phenol/water method - (Mar/27/2009 )
Resently, I want to isolate LPS from E.coli by hot phenol/water method which was described by Westphal, O. & Jann, K. (1965) Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. I have an ergent need to get it. Is there anybody who can help me to get this protocol? Thank you. 
heejun on Mar 27 2009, 08:18 AM said:
There was a similar topic like yours here a few years ago ( http://www.protocol-online.org/biology-for...sts/13636.html), however due to the servercrash this is lost,but maybe you should email Charlie Ellis: c.ellis@imperial.ac.uk on the matter. He has experience with the protocol you need, he also has the protocol, so he might be able to help you.
If he doenst answer you, you could try and email: Dr. Y. Veeranagouda at veeresha_y@rediffmail.com, he has also the protocol, I think.
I hope this helps.
pito on Mar 28 2009, 05:05 AM said:
heejun on Mar 27 2009, 08:18 AM said:
There was a similar topic like yours here a few years ago ( http://www.protocol-online.org/biology-for...sts/13636.html), however due to the servercrash this is lost,but maybe you should email Charlie Ellis: c.ellis@imperial.ac.uk on the matter. He has experience with the protocol you need, he also has the protocol, so he might be able to help you.
If he doenst answer you, you could try and email: Dr. Y. Veeranagouda at veeresha_y@rediffmail.com, he has also the protocol, I think.
I hope this helps.
Thank you very much. It's really very nice of you. And I will try to email the prof.
the link doesnt seem to work anymore and I can not edit the message thus here here is the corect link.
(http://www.protocol-online.org/biology-forums/posts/13636.html)
If it is possible heejun, could you then upload the protocol here or maybe send it to me?
thanks.
Anyone who reads this topic or needs info on the subject, be sure check the files attached.
