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LPS isolation by hot phenol\water method - (Feb/11/2006 )

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want isolate and purify bacterial lps by hot phenol\water method but I cant find this protocol .I need this protocol anybody can give me this protocol. thank you rolleyes.gif

-arf-

Hi arf,

I have done LPS extraction using the hot phenol-water method which was originally decribed by Westphal, O. & Jann, K. (1965) Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. Methods Carbohydr Chem 5: 83–91. I used a modification of it to extract Neisseria gonorrhoeae LPS (Mol Microbiol 2001 42: 167) which worked pretty well. If you need a more detailed protocol I could send you that.

Cheers,

Charlie.

-Charlie Ellis-

QUOTE (Charlie Ellis @ Feb 15 2006, 05:39 PM)
Hi arf,

I have done LPS extraction using the hot phenol-water method which was originally decribed by Westphal, O. & Jann, K. (1965) Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. Methods Carbohydr Chem 5: 83–91. I used a modification of it to extract Neisseria gonorrhoeae LPS (Mol Microbiol 2001 42: 167) which worked pretty well. If you need a more detailed protocol I could send you that.

Cheers,

Charlie.

Hi charlis Ellis
thank you for reply to my question. I need the protocol hot phenol\water decribed by Westphal, O. & Jann, K. (1965) Bacterial lipopolysaccharides.
please send me this protocol.
thanks lot.
best regard
ARF

-arf-

QUOTE (Charlie Ellis @ Feb 15 2006, 07:09 AM)
Hi arf,

I have done LPS extraction using the hot phenol-water method which was originally decribed by Westphal, O. & Jann, K. (1965) Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. Methods Carbohydr Chem 5: 83–91. I used a modification of it to extract Neisseria gonorrhoeae LPS (Mol Microbiol 2001 42: 167) which worked pretty well. If you need a more detailed protocol I could send you that.

Cheers,

Charlie.


hi charlie,
i am looking for protocol of hot phenol water extraction method described by westphal . would you be able to help me.

Neetu

-neetu-

QUOTE (Charlie Ellis @ Feb 15 2006, 07:09 AM)
Hi arf,

I have done LPS extraction using the hot phenol-water method which was originally decribed by Westphal, O. & Jann, K. (1965) Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. Methods Carbohydr Chem 5: 83–91. I used a modification of it to extract Neisseria gonorrhoeae LPS (Mol Microbiol 2001 42: 167) which worked pretty well. If you need a more detailed protocol I could send you that.

Cheers,

Charlie.

hi chailie

i need the article " hot phenol water method for LPS". can you send it for me?

THANK YOU

-kebin-

Hi Charlie,

I need this protocol too, could you send me please? thank you.

best regard,

wulan

I have done LPS extraction using the hot phenol-water method which was originally decribed by Westphal, O. & Jann, K. (1965) Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure. Methods Carbohydr Chem 5: 83–91. I used a modification of it to extract Neisseria gonorrhoeae LPS (Mol Microbiol 2001 42: 167) which worked pretty well. If you need a more detailed protocol I could send you that.

Cheers,

Charlie.
[/quote]

-wulandari dedy-

Dear All,

Please check your emails as I have sent you the information you have requested.

Thanks,

Charlie.

-Charlie Ellis-

Dear Charlie,
I need this protocol, too. Would you like send me one copy?


Thanks
Best wishes
lily_wn

-lily_wn-

Dear Charlie,
Could you sent me the protocol of lps isolation by hot phenol\water method?

with regards,
zss
2006.12.16

-zss-

Can you send one to me too Charlie?

Thanks.

-David Howards-
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