T lymphocyte ChIPs are not working... - while other cells are! *grumble grumble* (Mar/26/2009 )
Hi everyone
I'm stuck. For a while we have been trying to ChIP human T cells (sorted from peripheral blood using CD3 MACS beads) and it's not working When we ChIP cells from AML patients (not sorted) the ChIP works fine. We have tried ~6 different primer sets and the result is always the same. The T cell ChIPs do not work! ARG! I am going crazy! Why would all other chips work and not T cells? There must be something I have overlooked and it's driving me batty.
Any ideas??
Please???
I've always said "B cells are brilliant, T cells are trouble"
Clare
Clare on Mar 26 2009, 08:28 AM said:

I'm stuck. For a while we have been trying to ChIP human T cells (sorted from peripheral blood using CD3 MACS beads) and it's not working

Any ideas??
Please???
I've always said "B cells are brilliant, T cells are trouble"
Clare
Have you tried ChIP on any other sorted cells and had it work?
Hey there
Yes we have. We sorted human CD34+ cells using MACS beads and they work fine as well.
Hmmm..
Clare
PS: Happy Friday!
Have you tried ChIP on any other sorted cells and had it work?