Methods to determine the oligomeric state of a protein - (Mar/25/2009 )

Dear all,

I am currently working on a bacterial enzyme and would like to know its oligomeric state in vivo. I've found some methods such as performing cross-linking with dimethyl suberimidate or non-denaturing PAGE. I would like to ask if there are any other methods and which one would be a better one. And what would be the most preliminary method to check the oligomeric state?

Thanks in advance!

Hey,

Gel filtration chromatography, gluteraldehyde crosslinking, analytical ultracentrifugation.

Try GPC first.

Best,
TC